一种计算猪粪便中日本血吸虫卵的新技术。

A new technic for counting Schistosoma japonicum eggs in pig feces.

作者信息

Willingham A L, Johansen M V, Barnes E H

机构信息

Danish Center For Experimental Parasitology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.

出版信息

Southeast Asian J Trop Med Public Health. 1998 Mar;29(1):128-30.

PMID:9740285

Abstract

An improved laboratory method was developed for counting Schistosoma japonicum eggs in pig feces, which involves filtration, sedimentation and centrifugation, but avoids toxic chemicals. It is sensitive, allows easy differentiation from similar-sized and shaped protozoan cysts, and permits evaluation of egg viability both by direct viewing of eggs and miracidial hatching. It was found to be significantly better at recovering eggs than the modified Bell filtration technic. The sensitivity, specificity and practicality of this technic make it our method of choice for studies on porcine schistosomiasis japonica.

摘要

开发了一种改进的实验室方法用于计数猪粪便中的日本血吸虫卵，该方法包括过滤、沉淀和离心，但避免使用有毒化学物质。它灵敏度高，易于与大小和形状相似的原生动物囊肿区分开来，并且既可以通过直接观察虫卵又可以通过毛蚴孵化来评估虫卵的活力。结果发现，在回收虫卵方面，该方法比改良的贝尔过滤技术要好得多。该技术的灵敏度、特异性和实用性使其成为我们研究猪日本血吸虫病的首选方法。