通过Slack和Slo亚基相互作用形成中等电导钙激活钾通道。

Formation of intermediate-conductance calcium-activated potassium channels by interaction of Slack and Slo subunits.

作者信息

Joiner W J, Tang M D, Wang L Y, Dworetzky S I, Boissard C G, Gan L, Gribkoff V K, Kaczmarek L K

机构信息

Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

出版信息

Nat Neurosci. 1998 Oct;1(6):462-9. doi: 10.1038/2176.

DOI:10.1038/2176

PMID:10196543

Abstract

Large-conductance calcium-activated potassium channels (maxi-K channels) have an essential role in the control of excitability and secretion. Only one gene Slo is known to encode maxi-K channels, which are sensitive to both membrane potential and intracellular calcium. We have isolated a potassium channel gene called Slack that is abundantly expressed in the nervous system. Slack channels rectify outwardly with a unitary conductance of about 25-65 pS and are inhibited by intracellular calcium. However, when Slack is co-expressed with Slo, channels with pharmacological properties and single-channel conductances that do not match either Slack or Slo are formed. The Slack/Slo channels have intermediate conductances of about 60-180 pS and are activated by cytoplasmic calcium. Our findings indicate that some intermediate-conductance channels in the nervous system may result from an interaction between Slack and Slo channel subunits.

摘要

大电导钙激活钾通道（大钾通道）在控制兴奋性和分泌方面起着至关重要的作用。已知只有一个基因Slo编码大钾通道，该通道对膜电位和细胞内钙都敏感。我们分离出了一个名为Slack的钾通道基因，它在神经系统中大量表达。Slack通道向外整流，单位电导约为25 - 65 pS，并受到细胞内钙的抑制。然而，当Slack与Slo共表达时，会形成具有药理学特性和单通道电导的通道，这些特性与Slack或Slo都不匹配。Slack/Slo通道具有约60 - 180 pS的中间电导，并被细胞质钙激活。我们的研究结果表明，神经系统中的一些中间电导通道可能是由Slack和Slo通道亚基之间的相互作用产生的。