通过多态性DNA的随机扩增鉴定炭疽芽孢杆菌中的菌株特异性标记。
Identification of strain specific markers in Bacillus anthracis by random amplification of polymorphic DNA.
作者信息
Levy Haim, Fisher Morly, Ariel Naomi, Altboum Zeev, Kobiler David
机构信息
Department of Infectious Diseases, Israel Institute for Biological Research, PO Box 19, Ness-Ziona, Israel.
出版信息
FEMS Microbiol Lett. 2005 Mar 1;244(1):199-205. doi: 10.1016/j.femsle.2005.01.039.
Classification and differentiation of Bacillus anthracis isolates by genetic markers play an important role in anthrax research. We used a PCR based method--Random Amplification of Polymorphic DNA (RAPD)--to identify genetic markers in B. anthracis strains. Twenty-five differential genetic markers were identified which divided the strains into five different groups. Three selected RAPD-markers were cloned and sequenced. The five RAPD-derived genotypes could be defined by integration of these three markers. This system offers a simple non-expensive method to classify B. anthracis strains in laboratories involved in the research of this bacterium.
通过遗传标记对炭疽芽孢杆菌分离株进行分类和鉴别在炭疽研究中起着重要作用。我们使用了一种基于PCR的方法——随机扩增多态性DNA（RAPD）——来鉴定炭疽芽孢杆菌菌株中的遗传标记。共鉴定出25个差异遗传标记，这些标记将菌株分为五个不同的组。选取了三个RAPD标记进行克隆和测序。这五个由RAPD衍生的基因型可以通过整合这三个标记来定义。该系统为从事这种细菌研究的实验室提供了一种简单且低成本的方法来对炭疽芽孢杆菌菌株进行分类。
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