神经肽Y调节培养的青春期前小母猪垂体前叶细胞的生长激素分泌，但不调节促黄体生成素的分泌。

Neuropeptide Y modulates growth hormone but not luteinizing hormone secretion from prepuberal gilt anterior pituitary cells in culture.

作者信息

Barb C R, Barrett J B

机构信息

USDA-ARS, Animal Physiology Research Unit, Russell Agricultural Research Center, Athens, GA 30604-5677, USA.

出版信息

Domest Anim Endocrinol. 2005 Oct;29(3):548-55. doi: 10.1016/j.domaniend.2005.03.004. Epub 2005 Apr 7.

DOI:10.1016/j.domaniend.2005.03.004

PMID:16153503

Abstract

Pituitary cells, from seven 160- to 170-day-old pigs, were studied in primary culture to determine the affects NPY on LH and GH secretion at the level of the pituitary. On day 4 of culture, medium was discarded, plates were rinsed twice with serum-free medium and cells were cultured in 1 ml fresh medium without serum and challenged individually with 10(-10), 10(-8) or 10(-6) M [Ala(15)]-h growth hormone-releasing factor-(1-29)NH(2) (GRF); 10(-9), 10(-8) or 10(-7) M GnRH or 10(-9), 10(-8), 10(-7) or 10(-6) M NPY individually or in combinations with 10(-9) or 10(-8) M GnRH or 10(-8) or 10(-6)M GRF. Cells were exposed to treatment for 4 h at which time medium was harvested and quantified for LH and GH. Basal LH secretion (control; n = 7 pituitaries) was 12 +/- 6 ng/well. Relative to control at 4 h, 10(-9), 10(-8) and 10(-7) M GnRH increased (P < 0.01) LH secretion by 169, 176 and 197%, respectively. Neuropeptide-Y did not alter (P > 0.4) basal LH secretion nor 10(-8) M GnRH-induced increase in LH secretion but 10(-9) M GnRH-stimulated LH secretion was reduced by NPY and was not different from control or GnRH alone. Basal GH secretion (control; n = 7 pituitaries) was 56 +/- 12 ng/well. Relative to control at 4 h, 10(-10), 10(-8) and 10(-6) M GRF increased GH secretion by 111%, 125% (P < 0.01) and 150% (P < 0.01), respectively. Only 10(-6) M (134%) and 10(-7) M (125%) NPY increased (P < 0.04) basal GH secretion. Addition of 10(-9), 10(-8) and 10(-7) M NPY in combination with 10(-8) M GRF suppressed (P < 0.04) GRF-stimulated GH secretion. However, 10(-9) M NPY enhanced (P < 0.06) the GH response to 10(-6) M GRF. These results demonstrate that NPY may directly modulate GH secretion at the level of the pituitary gland.

摘要

对7头160至170日龄猪的垂体细胞进行原代培养，以确定神经肽Y（NPY）在垂体水平对促黄体生成素（LH）和生长激素（GH）分泌的影响。在培养的第4天，弃去培养基，用无血清培养基冲洗培养板两次，然后将细胞培养在1 ml无血清的新鲜培养基中，并分别用10⁻¹⁰、10⁻⁸或10⁻⁶ M [丙氨酸（¹⁵）]-人生长激素释放因子-（1 - 29）NH₂（GRF）；10⁻⁹、10⁻⁸或10⁻⁷ M促性腺激素释放激素（GnRH）或10⁻⁹、10⁻⁸、10⁻⁷或10⁻⁶ M NPY单独处理，或与10⁻⁹或10⁻⁸ M GnRH或10⁻⁸或10⁻⁶ M GRF联合处理。细胞暴露于处理4小时后，收集培养基并对LH和GH进行定量。基础LH分泌（对照组；n = 7个垂体）为12 ± 6 ng/孔。与4小时时的对照组相比，10⁻⁹、10⁻⁸和10⁻⁷ M GnRH分别使LH分泌增加（P < 0.01）169%、176%和197%。神经肽Y未改变（P > 0.4）基础LH分泌，也未改变10⁻⁸ M GnRH诱导的LH分泌增加，但10⁻⁹ M GnRH刺激的LH分泌被NPY降低，且与对照组或单独使用GnRH时无差异。基础GH分泌（对照组；n = 7个垂体）为56 ± 12 ng/孔。与4小时时的对照组相比，10⁻¹⁰、10⁻⁸和10⁻⁶ M GRF分别使GH分泌增加111%、125%（P < 0.01）和150%（P < 0.01）。仅10⁻⁶ M（134%）和10⁻⁷ M（125%）NPY增加（P < 0.04）基础GH分泌。将10⁻⁹、10⁻⁸和10⁻⁷ M NPY与10⁻⁸ M GRF联合使用可抑制（P < 0.04）GRF刺激的GH分泌。然而，10⁻⁹ M NPY增强（P < 0.06）了对10⁻⁶ M GRF的GH反应。这些结果表明，NPY可能在垂体水平直接调节GH分泌。