催产素单独及与特定下丘脑激素联合使用对发情周期母猪垂体前叶细胞促肾上腺皮质激素、β-内啡肽、促黄体生成素和催乳素分泌的影响。
Effects of oxytocin alone and in combination with selected hypothalamic hormones on ACTH, beta-endorphin, LH and PRL secretion by anterior pituitary cells of cyclic pigs.
作者信息
Kotwica Genowefa, Staszkiewicz Jarosław, Skowroński Mariusz T, Siawrys Gabriela, Bogacka Iwona, Franczak Anita, Kurowicka Beata, Kraziński Bartłomiej, Okrasa Stanisław
机构信息
Department of Animal Physiology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn-Kortowo, ul. Oczapowskiego 1A, Poland.
出版信息
Reprod Biol. 2006 Jul;6(2):115-31.
Oxytocin (OT) is involved in the stimulation of secretion of anterior pituitary hormones in females during the periovulatory and periparturient periods. In the present study we examined the role of OT in control of ACTH, beta-endorphin, LH and PRL secretion in vitro from dispersed anterior pituitary cells collected from gilts during the luteal (Days 10-12; n=6) and follicular (Days 18-20; n=5) phases of the estrous cycle. Isolated anterior pituitary cells (1 x 10(6)/ml) were transferred into 24-well plates, separately for each animal, and were pre-incubated for three days at 37 degrees C in atmosphere of 5% CO(2) and 95% air. The cells which attached to the dishes were incubated (3.5 h, 37 degrees C) in McCoy's medium in the absence (control) or in the presence of the following factors: CRH alone (10(-10), 10(-9), 10(-8), 10(-7) M), OT alone (10(-8), 10(-7), 10(-6) M), LVP alone (10(-7) M), OT (10(-7) M) plus CRH (10(-9) M) and LVP (10(-7) M) plus CRH (10(-9) M) for studying ACTH and beta-endorphin secretion; OT alone (10(-8), 10(-7), 10(-6) M), GnRH alone (100 ng/ml), CRH alone (10(-9) M), OT (10(-7) M) plus GnRH (100 ng/ml) and OT (10(-7) M) plus CRH (10(-9) M) for studying LH and PRL secretion. Concentrations of the studied hormones in media were analyzed by RIA. Oxytocin alone increased ACTH (at doses 10(-7), 10(-6) M), beta-endorphin (at dose 10(-8) M), LH (at dose 10(-8) M) and PRL (at doses 10(-7), 10(-6) M) secretion by pituitary cells isolated only from luteal-phase gilts. None of the studied hormone concentrations in the medium was increased in response to OT when pituitary cells of follicular-phase gilts were examined. Oxytocin in combination with CRH exerted an additive effect on beta-endorphin secretion during the luteal phase. Summarizing, in the present study the stimulatory effect of oxytocin on ACTH, beta-endorphin, LH and PRL secretion by pituitary cells isolated from gilts during the luteal phase was demonstrated. However, the cells collected from follicular-phase gilts appeared to be unresponsive to OT. Moreover, interaction between OT and CRH in affecting beta-endorphin secretion was shown. These results suggest that OT may be transiently involved in the modulation of anterior pituitary hormone secretion in cyclic pigs.
催产素（OT）在雌性动物排卵周期和围产期刺激垂体前叶激素的分泌。在本研究中，我们检测了OT对发情周期黄体期（第10 - 12天；n = 6）和卵泡期（第18 - 20天；n = 5）的后备母猪分离的垂体前叶细胞体外促肾上腺皮质激素（ACTH）、β-内啡肽、促黄体生成素（LH）和催乳素（PRL）分泌的控制作用。将分离的垂体前叶细胞（1×10⁶/ml）分别转移到24孔板中，每只动物一份，在37℃、5%二氧化碳和95%空气的环境中预孵育三天。贴壁细胞在 McCoy 培养基中孵育（3.5小时，37℃），分别在无（对照）或存在以下因素的情况下：单独的促肾上腺皮质激素释放激素（CRH，10⁻¹⁰、10⁻⁹、10⁻⁸、10⁻⁷ M）、单独的OT（10⁻⁸、10⁻⁷、10⁻⁶ M）、单独的赖氨酸加压素（LVP，10⁻⁷ M）、OT（10⁻⁷ M）加CRH（10⁻⁹ M）以及LVP（10⁻⁷ M）加CRH（10⁻⁹ M），用于研究ACTH和β-内啡肽的分泌；单独的OT（10⁻⁸、10⁻⁷、10⁻⁶ M）、单独的促性腺激素释放激素（GnRH，100 ng/ml）、单独的CRH（10⁻⁹ M）、OT（10⁻⁷ M）加GnRH（100 ng/ml）以及OT（10⁻⁷ M）加CRH（10⁻⁹ M），用于研究LH和PRL的分泌。通过放射免疫分析法（RIA）分析培养基中所研究激素的浓度。单独的OT可增加仅从黄体期后备母猪分离的垂体细胞ACTH（剂量为10⁻⁷、10⁻⁶ M）、β-内啡肽（剂量为10⁻⁸ M）、LH（剂量为10⁻⁸ M）和PRL（剂量为10⁻⁷、10⁻⁶ M）的分泌。当检测卵泡期后备母猪的垂体细胞时，培养基中所研究激素的浓度均未因OT而升高。在黄体期，OT与CRH联合对β-内啡肽的分泌具有相加作用。总之，在本研究中，证明了OT对黄体期后备母猪分离的垂体细胞ACTH、β-内啡肽、LH和PRL分泌具有刺激作用。然而，从卵泡期后备母猪收集的细胞似乎对OT无反应。此外，还显示了OT与CRH在影响β-内啡肽分泌方面的相互作用。这些结果表明，OT可能短暂参与了周期性母猪垂体前叶激素分泌的调节。
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