γ-干扰素激活的人单核细胞衍生巨噬细胞对荚膜组织胞浆菌的杀伤作用：超氧阴离子依赖性机制的证据。

Killing of Histoplasma capsulatum by gamma-interferon-activated human monocyte-derived macrophages: evidence for a superoxide anion-dependent mechanism.

作者信息

Brummer E, Kurita N, Yoshida S, Nishimura K, Miyaji M

机构信息

Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Japan.

出版信息

J Med Microbiol. 1991 Jul;35(1):29-34. doi: 10.1099/00222615-35-1-29.

DOI:10.1099/00222615-35-1-29

PMID:1649308

Abstract

The interaction of human macrophages with the yeast form of the thermally dimorphic fungal pathogen, Histoplasma capsulatum, was studied. Macrophages derived from monocytes by culture in vitro for 3 days ingested H. capsulatum, but were neither fungicidal or fungistatic. In contrast, when monocytes were exposed to human recombinant gamma-interferon (gamma-IFN) during their differentiation into macrophages, those macrophages were able to reduce the number of ingested or adherent cfu of H. capsulatum by 44-75% in 2 h. Activation of macrophages for fungicidal activity by gamma-IFN was dose dependent and 500-1000 units ml were optimal. Antibody to gamma-IFN abrogated the gamma-IFN activation process. Killing of H. capsulatum by activated macrophages in 2-h assays could be inhibited by superoxide dismutase but not by sodium azide.

摘要

研究了人类巨噬细胞与热双相真菌病原体荚膜组织胞浆菌酵母形式之间的相互作用。通过体外培养3天从单核细胞衍生而来的巨噬细胞摄取了荚膜组织胞浆菌，但既无杀真菌作用也无抑菌作用。相反，当单核细胞在分化为巨噬细胞的过程中暴露于人类重组γ干扰素（γ-IFN）时，这些巨噬细胞能够在2小时内将摄取或黏附的荚膜组织胞浆菌菌落形成单位数量减少44%-75%。γ-IFN激活巨噬细胞产生杀真菌活性呈剂量依赖性，500-1000单位/毫升最为适宜。γ-IFN抗体消除了γ-IFN激活过程。在2小时试验中，超氧化物歧化酶可抑制活化巨噬细胞对荚膜组织胞浆菌的杀伤作用，但叠氮化钠则无此作用。