越南胡志明市因腹泻住院的儿童中与急性胃肠炎相关的病毒多样性。

Diversity of viruses associated with acute gastroenteritis in children hospitalized with diarrhea in Ho Chi Minh City, Vietnam.

作者信息

Nguyen Tuan Anh, Yagyu Fumihiro, Okame Michio, Phan Tung Gia, Trinh Quang Duy, Yan Hainian, Hoang Kim Trong, Cao Anh Thi Huynh, Le Hoang Phuc, Okitsu Shoko, Ushijima Hiroshi

机构信息

Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

出版信息

J Med Virol. 2007 May;79(5):582-90. doi: 10.1002/jmv.20857.

DOI:10.1002/jmv.20857

PMID:17385670

Abstract

A molecular epidemiological study on common diarrheal viruses was conducted in Ho Chi Minh City, Vietnam between October 2002 and September 2003. Fecal samples were collected from 1,010 hospitalized children with acute gastroenteritis. Those samples were screened for groups A, B, and C rotavirus, adenovirus, genogroups I and II norovirus (NoV), sapovirus (SaV), and human astrovirus (HAstV) by RT-multiplex PCR, and the positive specimens were characterized further by ELISA, nested PCR, or sequencing. Among the diarrheal viruses detected, group A rotavirus was the most common, with a proportion of 67.4%, whereas NoV GII, adenovirus, SaV, and HAstV were also found in 5.5, 3.2, 0.8, and 0.6%, respectively. It is noteworthy that the group C rotavirus was first reported in Vietnam, with a proportion of 0.5% in this study. Fifty-six of 1,010 (5.5%) samples were found positive with more than one viral agent, in which 25 samples contained both group A rotavirus and NoV GII. Group A rotavirus could be identified throughout year with the peaks in both the dry and rainy season, whereas other viruses prevailed mainly in the rainy season. G-typing for the group A rotavirus showed that genotype 1 was still the most prevailing (33.0%), but interestingly, serotype 9 was emergent and became the third most common rotavirus G-type in these samples (13.7%). The four most common G-P combinations globally, G1P[8], G2P[4], G3P[8], and G4P[8] were found in 46.8% of rotavirus-positive samples, and it is of interest that one unusual rotavirus G9P[19] strain was first detected in Vietnam. The majority of NoV strains belonged to GII/4, and SaV strains mainly clustered with the Manchester strain (GI/1). Twenty-seven out of 32 adenovirus strains were identified as serotype 41. All HAstVs belonged to genotype 1. The results indicated clearly the impact of viral agents causing gastroenteritis and the importance of vaccination against diarrhea in Vietnam.

摘要

2002年10月至2003年9月期间，在越南胡志明市开展了一项关于常见腹泻病毒的分子流行病学研究。收集了1010例因急性肠胃炎住院儿童的粪便样本。通过逆转录多重聚合酶链反应（RT-multiplex PCR）对这些样本进行A、B、C组轮状病毒、腺病毒、I型和II型诺如病毒（NoV）、札如病毒（SaV）及人星状病毒（HAstV）筛查，对阳性标本进一步采用酶联免疫吸附测定（ELISA）、巢式聚合酶链反应或测序进行鉴定。在检测到的腹泻病毒中，A组轮状病毒最为常见，占比67.4%，而诺如病毒GII型、腺病毒、札如病毒和人星状病毒的检出率分别为5.5%、3.2%、0.8%和0.6%。值得注意的是，C组轮状病毒在越南首次被报道，本研究中的占比为0.5%。1010份样本中有56份（5.5%）被检测出感染了不止一种病毒，其中25份样本同时感染了A组轮状病毒和诺如病毒GII型。A组轮状病毒全年均可检出，在旱季和雨季均出现高峰，而其他病毒主要在雨季流行。对A组轮状病毒进行G分型显示，1型基因型仍然最为常见（33.0%），但有趣的是，9型血清型开始出现并成为这些样本中第三常见的轮状病毒G型（13.7%）。全球最常见的四种G-P组合，即G1P[8]、G2P[4]、G3P[8]和G4P[8]，在46.8%的轮状病毒阳性样本中被发现，有意思的是，越南首次检测到一株罕见的轮状病毒G9P[19]毒株。大多数诺如病毒毒株属于GII/4型，札如病毒毒株主要与曼彻斯特毒株（GI/1）聚类。32株腺病毒中有27株被鉴定为41型血清型。所有的人星状病毒均属于1型基因型。结果清楚地表明了病毒病原体对越南肠胃炎的影响以及腹泻疫苗接种的重要性。