Kirmizibekmez Hasan, Demirci Betül, Yeşilada Erdem, Başer K Hüsnü Can, Demirci Fatih
Department of Pharmacognosy, Faculty of Pharmacy, Yeditepe University, TR-34755, Kayişdaği, Istanbul, Turkey.
Nat Prod Commun. 2009 Jul;4(7):1001-6.
The chemical compositions of the essential oils obtained by hydrodistillation from the dried leaves and flowers of Lavandula stoechas L. ssp. stoechas were separately identified by GC-FID and GC-MS analyses. The main components were alpha-fenchone (41.9 +/- 1.2%), 1,8-cineole (15.6 +/- 0.8%), camphor (12.1 +/- 0.5%), and viridiflorol (4.1 +/- 0.4%) in the leaves; and alpha-fenchone (39.2 +/- 0.9%), myrtenyl acetate (9.5 +/- 0.4%), alpha-pinene (6.1 +/- 0.09%), camphor (5.9 +/- 0.05%) and 1,8-cineole (3.8 +/- 0.1%) in the flowers. Overall, 55 and 66 constituents were identified in the leaf and flower essential oils representing more than 90% and 94% of the total, respectively. In addition, the essential oils were evaluated for their antibacterial and anticandidal activities by broth microdilution. The flower essential oil was found to be relatively more active than the leaf oil towards the tested pathogenic microorganisms. Methicillin-resistant Staphylococcus aureus was more susceptible to the flower oil (MIC = 31.2 microg/mL). The oils, evaluated for their free radical scavenging activity using a TLC-DPPH assay, were inactive at a concentration of 2 mg/mL.
采用水蒸馏法从意大利薰衣草(Lavandula stoechas L. ssp. stoechas)的干燥叶片和花朵中提取挥发油,并通过气相色谱-火焰离子化检测器(GC-FID)和气相色谱-质谱联用仪(GC-MS)分析分别鉴定其化学成分。叶片中的主要成分是α-葑酮(41.9±1.2%)、1,8-桉叶素(15.6±0.8%)、樟脑(12.1±0.5%)和绿花白千层醇(4.1±0.4%);花朵中的主要成分是α-葑酮(39.2±0.9%)、桃金娘烯醇乙酸酯(9.5±0.4%)、α-蒎烯(6.1±0.09%)、樟脑(5.9±0.05%)和1,8-桉叶素(3.8±0.1%)。总体而言,在叶片和花朵挥发油中分别鉴定出55种和66种成分,分别占总量的90%以上和94%以上。此外,采用肉汤微量稀释法对挥发油的抗菌和抗念珠菌活性进行了评估。结果发现,花朵挥发油对受试致病微生物的活性相对高于叶片挥发油。耐甲氧西林金黄色葡萄球菌对花朵挥发油更敏感(最低抑菌浓度=31.2μg/mL)。采用薄层色谱-二苯基苦味酰基苯肼(TLC-DPPH)法对挥发油的自由基清除活性进行评估,结果表明,在浓度为2mg/mL时挥发油无活性。