磷酸化 Akt1 的表达与胰腺癌的预后良好相关。

Phosph-Akt1 expression is associated with a favourable prognosis in pancreatic cancer.

机构信息

Department of General Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, People's Republic of China.

出版信息

Ann Acad Med Singap. 2010 Jul;39(7):548-7.

PMID:20697673

Abstract

INTRODUCTION

Akt, a serine/threonine protein kinase, mediates growth factor-associated cell survival. In several human cancers, including pancreatic cancer, constitutive activation of Akt (phosphorylated Akt, p-Akt) has been observed and may be associated with chemotherapy and radiotherapy resistance. However, there are contradictory viewpoints in p-Akt in pancreatic cancer on prognosis, and the clinical relevance of p-Akt in pancreatic cancer is not well understood. This study aims to investigate the expressions and relevance of Akt and p-Akt1 in pancreatic cancer tissues and their clinical significance.

MATERIALS AND METHODS

The expressions of Akt and p-Akt in 74 surgically resected paraffin-embedded pancreatic ductal adenocarcinoma samples and 10 normal pancreatic tissue samples were examined by immunohistochemistry. The associations of their expression with clinicopathological and survival data were analysed.

RESULTS

The positive expression rate of Akt and p-Akt1 were 87.8% and 83.8%, respectively, which were remarkably higher then those in normal pancreatic tissue (P <0.05). There was a positive correlation between the expression of Akt and p-Akt1. High p-Akt1 expression correlated with lower T stage (P = 0.004), while Akt was not associated with any clinicopathologic variables. Kaplan-Meier survival analysis revealed that higher expression of Akt, p-Akt1 were respectively correlated with favourable prognosis (16.0[4.7-27.3] vs 9.3[9.0-9.6] months, P = 0.007, and 23.0[12.2-33.8] vs 11.1[7.5-14.7] months, P = 0.004, respectively). Multivariate analysis identified p-Akt1 as a significant independent favourable prognostic factor (HR=0.421, P = 0.010).

CONCLUSIONS

These results suggest that high p-Akt1 expression may be a favourable prognostic factor in pancreatic cancer.

摘要

简介

Akt 是一种丝氨酸/苏氨酸蛋白激酶，介导生长因子相关的细胞存活。在包括胰腺癌在内的几种人类癌症中，已经观察到 Akt 的组成性激活（磷酸化 Akt，p-Akt），并且可能与化疗和放疗抵抗有关。然而，在胰腺癌中 p-Akt 的预后存在矛盾观点，并且 p-Akt 在胰腺癌中的临床相关性尚不清楚。本研究旨在研究 Akt 和 p-Akt1 在胰腺癌细胞中的表达及其相关性，并探讨其临床意义。

材料和方法

通过免疫组织化学法检测 74 例手术切除的石蜡包埋胰腺导管腺癌样本和 10 例正常胰腺组织样本中 Akt 和 p-Akt1 的表达。分析其表达与临床病理和生存数据的相关性。

结果

Akt 和 p-Akt1 的阳性表达率分别为 87.8%和 83.8%，明显高于正常胰腺组织（P<0.05）。Akt 和 p-Akt1 的表达呈正相关。p-Akt1 的高表达与较低的 T 分期相关（P=0.004），而 Akt 与任何临床病理变量均无关。Kaplan-Meier 生存分析显示，Akt 和 p-Akt1 的高表达分别与良好的预后相关（16.0[4.7-27.3] vs 9.3[9.0-9.6] 个月，P=0.007，和 23.0[12.2-33.8] vs 11.1[7.5-14.7] 个月，P=0.004）。多因素分析表明 p-Akt1 是一个显著的独立预后良好因素（HR=0.421，P=0.010）。