肺炎克雷伯菌 LamB 孔蛋白在抗菌药物耐药性中的作用。
Role of Klebsiella pneumoniae LamB Porin in antimicrobial resistance.
机构信息
Instituto Universitario de Investigaciones en Ciencias de Salud, Universidad de Islas Baleares, Palma de Mallorca, Spain.
出版信息
Antimicrob Agents Chemother. 2011 Apr;55(4):1803-5. doi: 10.1128/AAC.01441-10. Epub 2011 Jan 31.
Abstract
To investigate the contribution of LamB in Klebsiella pneumoniae antimicrobial resistance, we determined the MICs of various antibiotics and the frequency of mutation to increased cefoxitin or meropenem resistance of the strains CSUB10S (expressing only OmpK36), CSUB10R (lacking OmpK35 and OmpK36), and their derived isogenic insertion-duplication mutants deficient in LamB. Expression of LamB was indispensable in order for CSUB10S to lose OmpK36 and become resistant to cefoxitin, while in CSUB10R, LamB deficiency promoted increased resistance to carbapenem.
摘要
为了研究 LamB 在肺炎克雷伯菌抗药性中的作用，我们测定了各抗生素的 MIC 值以及 CSUB10S 株（仅表达 OmpK36）、CSUB10R 株（缺乏 OmpK35 和 OmpK36）及其衍生的同源插入缺失突变体对增加头孢西丁或美罗培南耐药性的突变频率。LamB 的表达对于 CSUB10S 丢失 OmpK36 并对头孢西丁产生耐药性是不可或缺的，而在 CSUB10R 中，LamB 缺失促进了对碳青霉烯类药物的耐药性增加。
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