镉胁迫下多年生黑麦草（Lolium perenne L.）的抗氧化反应和基因表达。
Antioxidant responses and gene expression in perennial ryegrass (Lolium perenne L.) under cadmium stress.
机构信息
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Science, Wuhan City, 430074, Hubei, People's Republic of China.
出版信息
Ecotoxicology. 2011 Jun;20(4):770-8. doi: 10.1007/s10646-011-0628-y. Epub 2011 Mar 26.
Perennial ryegrass (Lolium perenne L.), widely used in temperate climates, is one of turf grasses that enrich cadmium (Cd). The objective of this study was to explore the physiological responses and candidate gene expression in perennial ryegrass to Cd stress. Grasses were subjected to three levels of 0, 0.2, and 0.5 mM Cd for 7 days in the greenhouse. The results indicated that soluble protein content was lower in the Cd-treated perennial ryegrass compared to untreated plants. The Cd-treated perennial ryegrass exhibited a greater level of malondialdehyde and activity of the peroxidase (POD), catalase, and superoxide dismutase (SOD) relative to the control. The Cd stress induced up-regulated expression of FeSOD, MnSOD, Chl Cu/ZnSOD, Cyt Cu/ZnSOD, APX, GPX, GR and POD at 4-24 h after treatment began for perennial ryegrass. Results suggested that the gene transcript profile was related to the enzyme activity under Cd stress.
多年生黑麦草（Lolium perenne L.）广泛用于温带地区，是一种富镉的草坪草。本研究旨在探讨镉胁迫下多年生黑麦草的生理响应和候选基因表达。在温室中，将草置于 0、0.2 和 0.5 mM Cd 三个水平下处理 7 天。结果表明，与未处理的植物相比，Cd 处理的多年生黑麦草中的可溶性蛋白含量较低。与对照相比，Cd 处理的多年生黑麦草的丙二醛含量和过氧化物酶（POD）、过氧化氢酶和超氧化物歧化酶（SOD）的活性更高。Cd 胁迫诱导多年生黑麦草在处理开始后 4-24 小时内上调 FeSOD、MnSOD、Chl Cu/ZnSOD、Cyt Cu/ZnSOD、APX、GPX、GR 和 POD 的表达。结果表明，基因转录谱与 Cd 胁迫下的酶活性有关。
Zotero 插件