使用微球菌核酸酶测序（MNase-Seq）确定后生动物中核小体占据情况的全基因组方法。
Genome-wide approaches to determining nucleosome occupancy in metazoans using MNase-Seq.
作者信息
Cui Kairong, Zhao Keji
机构信息
Laboratory of Molecular Immunology, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
出版信息
Methods Mol Biol. 2012;833:413-9. doi: 10.1007/978-1-61779-477-3_24.
Abstract
The precise location of nucleosomes in functional regulatory regions in chromatin is critical to the regulation of transcription. The nucleosome structure protects DNA from microccocal nuclease (MNase) digestion and leaves a footprint on DNA that indicates the position of nucleosomes. Short sequence reads (25-36 bp) from ends of mononucleosome-sized DNA generated from MNase digestion of chromatin can be determined using next-generation sequencing techniques. Mapping of these short reads to the genome provides a powerful genome-wide approach to precisely define the nucleosome positions in any genome with known genomic sequence. This chapter outlines the reagents and experimental procedures of MNase-Seq for mapping nucleosome positions in the human genome.
摘要
染色质功能调控区域中核小体的精确位置对于转录调控至关重要。核小体结构可保护DNA免受微球菌核酸酶（MNase）消化，并在DNA上留下表明核小体位置的足迹。使用下一代测序技术可以确定由染色质MNase消化产生的单核小体大小DNA末端的短序列读数（25 - 36 bp）。将这些短读数定位到基因组提供了一种强大的全基因组方法，可精确确定任何具有已知基因组序列的基因组中的核小体位置。本章概述了用于绘制人类基因组中核小体位置的MNase-Seq试剂和实验程序。
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