人乳头瘤病毒E6、E7 mRNA流式细胞术检测与人乳头瘤病毒DNA分型的临床性能比较
Clinical performance of human papillomavirus E6, E7 mRNA flow cytometric assay compared to human papillomavirus DNA typing.
作者信息
Kottaridi Christine, Tsiodras Sotirios, Spathis Aris, Chranioti Aikaterini, Pappas Asimakis, Kassanos Dimitrios, Panayiotides Ioannis, Karakitsos Petros
机构信息
Department of Cytopathology, Attikon University Hospital, Athens University Medical School, 1 Rimini Street, 12462 Chaidari, Athens, Greece.
出版信息
Anal Quant Cytol Histol. 2011 Dec;33(6):305-10.
OBJECTIVE
To use flow cytometry to screen cervical samples for the overexpression of human papillomavirus (HPV) E6 and E7 mRNA and compare the performance of this assay with an HPV DNA array for the detection of high-grade cervical lesions.
STUDY DESIGN
Cervical samples were analyzed for HPV DNA by clinical arrays, and the overexpression of E6 and E7 viral oncogenes was monitored using an HPV mRNA detection kit that quantifies the intracellular HPV E6 and E7 mRNA on a cell-by-cell basis.
RESULTS
HPV positivity increased with severity of histologic lesions. On the basis of histology-confirmed CIN 2+ cases the specificity of HPV assay was 73.9% (95% CI 66.07, 80.88), whereas it was 39.3% (95% CI 31.85, 47.1) for the DNA assay.
CONCLUSION
The HPV assay provides an early predictor of persistent HPV infection and may improve cervical cancer screening by increasing the specificity of detecting high-grade lesions.
目的
运用流式细胞术筛查宫颈样本中人乳头瘤病毒（HPV）E6和E7 mRNA的过表达情况，并将该检测方法与HPV DNA芯片检测高级别宫颈病变的性能进行比较。
研究设计
通过临床芯片分析宫颈样本中的HPV DNA，并使用HPV mRNA检测试剂盒监测E6和E7病毒癌基因的过表达情况，该试剂盒可逐细胞定量细胞内HPV E6和E7 mRNA。
结果
HPV阳性率随组织学病变严重程度增加。基于组织学确诊的CIN 2+病例，HPV检测的特异性为73.9%（95%可信区间66.07, 80.88），而DNA检测的特异性为39.3%（95%可信区间31.85, 47.1）。
结论
HPV检测可提供持续性HPV感染的早期预测指标，通过提高检测高级别病变的特异性，可能改善宫颈癌筛查。
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