补肾壮筋汤含药血清对力学刺激诱导软骨细胞凋亡的影响
[Effects of Bushen Zhuangjin Decoction containing serum on the apoptosis of chondrocytes induced by mechanics stimulus].
作者信息
Zhou Guo-Shun, Li Xiong-Feng, Guan Guo-Hua
机构信息
Department of Orthopedics, Huzhou Central Hospital of Zhejiang Province, Zhejiang.
出版信息
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2012 Jun;32(6):789-92.
OBJECTIVE
To study the effects of Bushen Zhuangjin Decoction (BZD) containing serum on the apoptosis of chondrocytes induced by mechanics stimulus.
METHODS
The BZD containing serum was extracted. The chondrocyte nutritive media was divided into 3 groups, i.e., the common nutritive medium group, the blank rabbit serum medium group, and the BZD nutritive medium group. The apoptosis of chondrocytes was induced by continuing mechanics stimulus in 24 h. Then the chondrocytes were collected. The apoptosis rate of chondrocytes was determined by flow cytometry. The contents of interleukin 1beta (IL-1beta) and nitric oxide (NO) in the corresponding media were determined.
RESULTS
The apoptosis of chondrocytes in the BZD nutritive medium group (19.55 +/- 7.98)% was lower than that of the common nutritive medium group (39.32 +/- 13.45)% and the blank rabbit serum medium group (37.87 +/- 9.67)%, showing statistical difference (P < 0.05). The contents of IL-1beta and NO were also lower in the BZD nutritive medium group with statistical difference when compared with those of the other two groups (P < 0.05).
CONCLUSION
BZD containing serum could protect mechanics stimulus induced apoptosis of chondrocytes.
目的
研究补肾壮筋汤含药血清对力学刺激诱导的软骨细胞凋亡的影响。
方法
制备补肾壮筋汤含药血清。将软骨细胞培养液分为3组，即普通培养液组、空白兔血清培养液组和补肾壮筋汤培养液组。通过持续24小时的力学刺激诱导软骨细胞凋亡。然后收集软骨细胞。采用流式细胞术检测软骨细胞凋亡率。测定相应培养液中白细胞介素1β（IL-1β）和一氧化氮（NO）的含量。
结果
补肾壮筋汤培养液组软骨细胞凋亡率为（19.55±7.98）%，低于普通培养液组（39.32±13.45）%和空白兔血清培养液组（37.87±9.67）%，差异有统计学意义（P<0.05）。补肾壮筋汤培养液组IL-1β和NO含量也低于其他两组，差异有统计学意义（P<0.05）。
结论
补肾壮筋汤含药血清可保护力学刺激诱导的软骨细胞凋亡。
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