基于等温循环链置换聚合反应和氧化石墨烯的无标记放大荧光 DNA 检测。
A label-free amplified fluorescence DNA detection based on isothermal circular strand-displacement polymerization reaction and graphene oxide.
机构信息
State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.
出版信息
Analyst. 2013 Jul 7;138(13):3616-20. doi: 10.1039/c3an00421j.
PMID:23671905
Abstract
A label-free fluorescent DNA biosensor has been presented based on isothermal circular strand-displacement polymerization reaction (ICSDPR) combined with graphene oxide (GO) binding. The proposed method is simple and cost-effective with a low detection limit of 4 pM, which compares favorably with other GO-based homogenous DNA detection methods.
摘要
一种基于等温循环链置换聚合反应（ICSDPR）与氧化石墨烯（GO）结合的无标记荧光 DNA 生物传感器已经被提出。该方法简单且具有成本效益，检测限低至 4 pM，优于其他基于 GO 的均相 DNA 检测方法。
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