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一种基于三价铕离子的 ATP 选择性近红外比率型荧光探针的构建

An ATP-selective, lanthanide complex luminescent probe.

机构信息

State Key Laboratory of Applied Organic Chemistry and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, PR China.

出版信息

Dalton Trans. 2013 Jul 21;42(27):9840-6. doi: 10.1039/c3dt50986a. Epub 2013 May 20.

Abstract

A luminescent probe based on a europium complex is developed, which effectively distinguishes adenosine-5'-triphosphate (ATP) from adenosine diphosphate (ADP) and adenosine monophosphate (AMP) in pure water at pH 6.8. With a longer lifetime (in ms range), the probe is prospectively applied to biological systems to monitor ATP levels by completely removing the background fluorescence of other molecules.

摘要

开发了一种基于铕配合物的荧光探针,该探针在 pH 值为 6.8 的纯水中可有效区分三磷酸腺苷 (ATP)、二磷酸腺苷 (ADP) 和一磷酸腺苷 (AMP)。该探针具有较长的寿命(毫秒级),可用于生物体系,通过完全去除其他分子的背景荧光来监测 ATP 水平。

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