伊朗绵羊流产中犬新孢子虫的检测
Detection of Neospora caninum in ovine abortion in Iran.
作者信息
Asadpour R, Jafari-Joozani R, Salehi N
机构信息
Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
出版信息
J Parasit Dis. 2013 Apr;37(1):105-9. doi: 10.1007/s12639-012-0141-0. Epub 2012 Jul 17.
The present study was designed to assess the importance of ovine neosporosis in abortion of Iraninan sheep. Seventy aborted foetuses and dams from ovine dairy farms in northwest of Iran were analyzed to investigate the role of Neospora caninum (N. caninum) in ovine abortion. Diagnosis of the infection was determined by serology and polymerase chain reaction (PCR). A total of 70 aborted dairy ovine were blood sampled and used to evaluate serological status for N. caninum infection by enzyme-linked immunosorbent assay (ELISA) and extracted DNA from the same aborted foetuses were subjected to PCR. Data were compared using Kruscal-Wallis test. From A total of the 70 sheeps, four (5.7 %) of the dams were seropositive. DNA from aborted foetuses was extracted primarily from placenta and CNS tissues. Extracted DNA from foetuses were analyzed using PCR with primers Np21(+) and Np6(+). Out of the 70 ovine fetuses 8.5 % were considered to be infected by PCR. This study confirms the importance of N. caninum as an important cause of ovine abortion in northwest of Iran.
本研究旨在评估绵羊新孢子虫病在伊朗绵羊流产中的重要性。对伊朗西北部绵羊奶牛场的70例流产胎儿和母羊进行分析，以调查犬新孢子虫（N. caninum）在绵羊流产中的作用。通过血清学和聚合酶链反应（PCR）确定感染的诊断。共采集了70例流产奶牛羊的血液样本，用于通过酶联免疫吸附测定（ELISA）评估犬新孢子虫感染的血清学状态，并对同一流产胎儿提取的DNA进行PCR检测。使用Kruskal-Wallis检验比较数据。在总共70只绵羊中，有4只（5.7%）母羊血清呈阳性。流产胎儿的DNA主要从胎盘和中枢神经系统组织中提取。使用引物Np21(+)和Np6(+)通过PCR分析从胎儿中提取的DNA。在70例绵羊胎儿中，8.5%被认为通过PCR检测感染。本研究证实了犬新孢子虫作为伊朗西北部绵羊流产重要病因的重要性。
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