超分辨率显微镜简介。

Introduction to super-resolution microscopy.

作者信息

Yamanaka Masahito, Smith Nicholas I, Fujita Katsumasa

机构信息

Department of Applied Physics Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan.

Department of Applied Physics Immunology Frontier Research Center, Osaka University, Suita, Osaka, Japan

出版信息

Microscopy (Oxf). 2014 Jun;63(3):177-92. doi: 10.1093/jmicro/dfu007. Epub 2014 Mar 25.

DOI:10.1093/jmicro/dfu007

PMID:24671128

Abstract

In this review, we introduce the principles of spatial resolution improvement in super-resolution microscopies that were recently developed. These super-resolution techniques utilize the interaction of light and fluorescent probes in order to break the diffraction barrier that limits spatial resolution. The imaging property of each super-resolution technique is also compared with the corresponding conventional one. Typical applications of the super-resolution techniques in biological research are also introduced.

摘要

在本综述中，我们介绍了最近开发的超分辨率显微镜中提高空间分辨率的原理。这些超分辨率技术利用光与荧光探针的相互作用来突破限制空间分辨率的衍射极限。还将每种超分辨率技术的成像特性与相应的传统技术进行了比较。此外，还介绍了超分辨率技术在生物学研究中的典型应用。