谷氨酰胺可预防肠系膜缺血再灌注模型中的氧化应激。
Glutamine prevents oxidative stress in a model of mesenteric ischemia and reperfusion.
作者信息
Zabot Gilmara Pandolfo, Carvalhal Gustavo Franco, Marroni Norma Possa, Hartmann Renata Minuzzo, da Silva Vinícius Duval, Fillmann Henrique Sarubbi
机构信息
Gilmara Pandolfo Zabot, Gustavo Franco Carvalhal, Henrique Sarubbi Fillmann, Department of Surgery, School of Medicine, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS 90610-000, Brazil.
出版信息
World J Gastroenterol. 2014 Aug 28;20(32):11406-14. doi: 10.3748/wjg.v20.i32.11406.
AIM
To evaluate preventative effects of glutamine in an animal model of gut ischemia/reperfusion (I/R).
METHODS
Male Wistar rats were housed in a controlled environment and allowed access to food and water ad libitum. Twenty male Wistar rats were divided into four experimental groups: (1) control group (control) - rats underwent exploratory laparotomy; (2) control + glutamine group (control-GLU) - rats were subjected to laparotomy and treated intraperitoneally with glutamine 24 and 48 h prior to surgery; (3) I/R group - rats were subjected to occlusion of the superior mesenteric artery for 30 min followed by 15 min of reperfusion; and (4) ischemia/reperfusion + glutamine group (G + I/R) - rats were treated intraperitoneally with glutamine 24 and 48 h before I/R. Local and systemic injuries were determined by evaluating intestinal and lung segments for oxidative stress using lipid peroxidation and the activity of superoxide dismutase (SOD), interleukin-6 (IL-6) and nuclear factor kappa beta (NF-κB) after mesenteric I/R.
RESULTS
Lipid peroxidation of the membrane was increased in the animals subjected to I/R (P < 0.05). However, the group that received glutamine 24 and 48 h before the I/R procedure showed levels of lipid peroxidation similar to the control groups (P < 0.05). The activity of the antioxidant enzyme SOD was decreased in the gut of animals subjected to I/R when compared with the control group of animals not subjected to I/R (P < 0.05). However, the group that received glutamine 24 and 48 h before I/R showed similar SOD activity to both control groups not subjected to I/R (P < 0.05). The mean area of NF-κB staining for each of the control groups was similar. The I/R group showed the largest area of staining for NF-κB. The G + I/R group had the second highest amount of staining, but the mean value was much lower than that of the I/R group (P < 0.05). For IL-6, control and control-GLU groups showed similar areas of staining. The I/R group contained the largest area of IL-6 staining, followed by the G + I/R animals; however, this area was significantly lower than that of the group that underwent I/R without glutamine (P < 0.05).
CONCLUSION
These results demonstrate that pretreatment with glutamine prevents mucosal injury and improves gut and lung recovery after I/R injury in rats.
目的
评估谷氨酰胺在肠道缺血/再灌注（I/R）动物模型中的预防作用。
方法
雄性Wistar大鼠饲养在可控环境中，自由获取食物和水。20只雄性Wistar大鼠分为四个实验组：（1）对照组（control）——大鼠接受剖腹探查术；（2）对照组+谷氨酰胺组（control-GLU）——大鼠接受剖腹术，并在手术前24小时和48小时腹腔注射谷氨酰胺；（3）I/R组——大鼠肠系膜上动脉闭塞30分钟，随后再灌注15分钟；（4）缺血/再灌注+谷氨酰胺组（G + I/R）——大鼠在I/R前24小时和48小时腹腔注射谷氨酰胺。通过评估肠系膜I/R后肠段和肺段的脂质过氧化、超氧化物歧化酶（SOD）、白细胞介素-6（IL-6）和核因子κB（NF-κB）的活性来确定局部和全身损伤。
结果
I/R组动物的膜脂质过氧化增加（P < 0.05）。然而，在I/R手术前24小时和48小时接受谷氨酰胺的组，其脂质过氧化水平与对照组相似（P < 0.05）。与未接受I/R的对照组动物相比，I/R组动物肠道中抗氧化酶SOD的活性降低（P < 0.05）。然而，在I/R前24小时和48小时接受谷氨酰胺的组，其SOD活性与未接受I/R的两个对照组相似（P < 0.05）。每个对照组的NF-κB染色平均面积相似。I/R组的NF-κB染色面积最大。G + I/R组的染色量第二高，但平均值远低于I/R组（P < 0.05）。对于IL-6，对照组和control-GLU组的染色面积相似。I/R组的IL-6染色面积最大，其次是G + I/R组动物；然而，该面积显著低于未使用谷氨酰胺进行I/R的组（P < 0.05）。
结论
这些结果表明，谷氨酰胺预处理可预防大鼠I/R损伤后的黏膜损伤，并改善肠道和肺的恢复。
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