四氯化碳诱导大鼠遗传毒性和DNA氧化损伤：苣荬菜的保肝作用
CCl4 induced genotoxicity and DNA oxidative damages in rats: hepatoprotective effect of Sonchus arvensis.
作者信息
Alkreathy Huda Mohammad, Khan Rahmat Ali, Khan Muhammad Rashid, Sahreen Sumaira
机构信息
Pharmacology Department, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudia Arabia.
出版信息
BMC Complement Altern Med. 2014 Nov 21;14:452. doi: 10.1186/1472-6882-14-452.
BACKGROUND
Sonchus arvesis is traditionally reported in various human ailments including hepatotoxicity in Pakistan. Presently we designed to assess the protective effects of methanolic extract of Sonchus arvesis against carbon tetrachloride induced genotoxicity and DNA oxidative damages in hepatic tissues of experimental rats.
METHODS
36 male Sprague-Dawley rats were randomly divided into 6 groups to evaluate the hepatoprotective effects of Sonchus arvensis against CCl4 induced genotoxicity, DNA damages and antioxidant depletion. Rats of normal control group were given free access of food and water add labitum. Group II rats received 3 ml/kg of CCl4 (30% in olive oil v/v) via the intraperitoneal route twice a week for four weeks. Group III and IV received 1 ml of 100 mg/kg b.w. and 200 mg/kg b.w. SME via gavage after 48 h of CCl4 treatment whereas group V was given 1 ml of silymarin (100 mg/kg b.w.) after 48 h of CCl4 treatment. Group VI only received 200 mg/kg b.w. SME. Protective effects of SME were checked by measuring serum markers, activities of antioxidant enzymes, genotoxicity and DNA dmages.
RESULTS
Results of the present study showed that treatment of SME reversed the activities of serum marker enzymes and cholesterol profile as depleted with CCl4 treatment. Activities of endogenous antioxidant enzymes of liver tissue homogenate; catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSHpx), glutathione-S-transferase (GST) and glutathione reductase (GSR) were reduced with administration of CCl4, which were returned to the control level with SME treatment. CCl4-induced hepatic cirrhosis decreased hepatic glutathione (GSH) and increased lipid peroxidative products (TBARS), were normalized by treatment with SME. Moreover, administration of CCl4 caused genotoxicity and DNA fragmentation which were significantly restored towards the normal level with SME.
CONCLUSION
These results reveal that treatment of SME may be useful in the prevention of hepatic stress.
背景
在巴基斯坦，传统医学报道苣荬菜可用于治疗多种人类疾病，包括肝毒性。目前，我们旨在评估苣荬菜甲醇提取物对四氯化碳诱导的实验大鼠肝组织遗传毒性和DNA氧化损伤的保护作用。
方法
将36只雄性Sprague-Dawley大鼠随机分为6组，以评估苣荬菜对四氯化碳诱导的遗传毒性、DNA损伤和抗氧化剂耗竭的肝保护作用。正常对照组大鼠自由摄取食物和水。第二组大鼠每周经腹腔注射两次3 ml/kg的四氯化碳（30%溶于橄榄油，v/v），持续四周。第三组和第四组在四氯化碳处理48小时后，通过灌胃给予1 ml 100 mg/kg体重和200 mg/kg体重的苣荬菜甲醇提取物，而第五组在四氯化碳处理48小时后给予1 ml水飞蓟宾（100 mg/kg体重）。第六组仅给予200 mg/kg体重的苣荬菜甲醇提取物。通过测量血清标志物、抗氧化酶活性、遗传毒性和DNA损伤来检查苣荬菜甲醇提取物的保护作用。
结果
本研究结果表明，苣荬菜甲醇提取物的治疗逆转了血清标志物酶的活性和四氯化碳处理后降低的胆固醇水平。肝组织匀浆中内源性抗氧化酶的活性；过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSHpx）、谷胱甘肽-S-转移酶（GST）和谷胱甘肽还原酶（GSR）在给予四氯化碳后降低，而苣荬菜甲醇提取物治疗使其恢复到对照水平。四氯化碳诱导的肝硬化降低了肝谷胱甘肽（GSH）并增加了脂质过氧化产物（TBARS），经苣荬菜甲醇提取物治疗后恢复正常。此外，给予四氯化碳导致遗传毒性和DNA片段化，而苣荬菜甲醇提取物使其显著恢复到正常水平。
结论
这些结果表明，苣荬菜甲醇提取物的治疗可能有助于预防肝脏应激。
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