受压蠕虫：秀丽隐杆线虫的整体力学特性与角质层无关。
Worms under Pressure: Bulk Mechanical Properties of C. elegans Are Independent of the Cuticle.
作者信息
Gilpin William, Uppaluri Sravanti, Brangwynne Clifford P
机构信息
Department of Physics, Princeton University, Princeton, New Jersey.
Department of Chemical and Biological Engineering, Princeton University, Princeton, New Jersey.
出版信息
Biophys J. 2015 Apr 21;108(8):1887-98. doi: 10.1016/j.bpj.2015.03.020.
The mechanical properties of cells and tissues play a well-known role in physiology and disease. The model organism Caenorhabditis elegans exhibits mechanical properties that are still poorly understood, but are thought to be dominated by its collagen-rich outer cuticle. To our knowledge, we use a novel microfluidic technique to reveal that the worm responds linearly to low applied hydrostatic stress, exhibiting a volumetric compression with a bulk modulus, κ = 140 ± 20 kPa; applying negative pressures leads to volumetric expansion of the worm, with a similar bulk modulus. Surprisingly, however, we find that a variety of collagen mutants and pharmacological perturbations targeting the cuticle do not impact the bulk modulus. Moreover, the worm exhibits dramatic stiffening at higher stresses-behavior that is also independent of the cuticle. The stress-strain curves for all conditions can be scaled onto a master equation, suggesting that C. elegans exhibits a universal elastic response dominated by the mechanics of pressurized internal organs.
细胞和组织的力学特性在生理学和疾病中发挥着众所周知的作用。模式生物秀丽隐杆线虫表现出的力学特性仍未得到充分了解，但人们认为其主要由富含胶原蛋白的外皮决定。据我们所知，我们使用一种新型微流控技术揭示，线虫对低施加静水压力呈线性响应，表现出具有体积模量κ = 140 ± 20 kPa的体积压缩；施加负压会导致线虫体积膨胀，体积模量相似。然而，令人惊讶的是，我们发现各种胶原蛋白突变体和针对外皮的药理学扰动并不会影响体积模量。此外，线虫在较高应力下表现出显著的硬化——这种行为也与外皮无关。所有条件下的应力-应变曲线都可以缩放到一个主方程上，这表明秀丽隐杆线虫表现出一种由受压内部器官的力学主导的通用弹性响应。
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