灵长类胚胎中的靶向基因组编辑。
Targeted genome editing in primate embryos.
作者信息
Guo Xiangyu, Li Xiao-Jiang
机构信息
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
1] Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China [2] Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA.
出版信息
Cell Res. 2015 Jul;25(7):767-8. doi: 10.1038/cr.2015.64. Epub 2015 Jun 2.
Abstract
Mosaic mutations and off-target effects caused by CRISPR/Cas9 have led to concerns about the efficiency and specificity of this new technique in non-human primates and other large animals. Here we discuss recent findings from primate embryos, with a focus on the technical issues CRISPR/Cas9 faces before producing non-human primate models of human diseases.
摘要
CRISPR/Cas9引发的嵌合突变和脱靶效应引发了人们对这项新技术在非人类灵长类动物和其他大型动物中的效率和特异性的担忧。在此，我们讨论了灵长类胚胎的最新研究结果，重点关注CRISPR/Cas9在构建人类疾病的非人类灵长类动物模型之前所面临的技术问题。
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