EMRinger：用于三维冷冻电子显微镜的侧链导向模型与图谱验证
EMRinger: side chain-directed model and map validation for 3D cryo-electron microscopy.
作者信息
Barad Benjamin A, Echols Nathaniel, Wang Ray Yu-Ruei, Cheng Yifan, DiMaio Frank, Adams Paul D, Fraser James S
机构信息
Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
Graduate Group in Biophysics, University of California, San Francisco, San Francisco, California, USA.
出版信息
Nat Methods. 2015 Oct;12(10):943-6. doi: 10.1038/nmeth.3541. Epub 2015 Aug 17.
Advances in high-resolution cryo-electron microscopy (cryo-EM) require the development of validation metrics to independently assess map quality and model geometry. We report EMRinger, a tool that assesses the precise fitting of an atomic model into the map during refinement and shows how radiation damage alters scattering from negatively charged amino acids. EMRinger (https://github.com/fraser-lab/EMRinger) will be useful for monitoring progress in resolving and modeling high-resolution features in cryo-EM.
高分辨率冷冻电子显微镜（cryo-EM）技术的进步需要开发验证指标，以独立评估图谱质量和模型几何结构。我们报告了EMRinger，这是一种在精修过程中评估原子模型与图谱精确拟合度的工具，并展示了辐射损伤如何改变带负电荷氨基酸的散射。EMRinger（https://github.com/fraser-lab/EMRinger）将有助于监测在解析和建模冷冻电子显微镜高分辨率特征方面的进展。
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