鱼油给药通过调节p53、Bcl-2、半胱天冬酶-7和半胱天冬酶-3蛋白表达介导Walker 256肿瘤细胞凋亡。
Fish oil administration mediates apoptosis of Walker 256 tumor cells by modulation of p53, Bcl-2, caspase-7 and caspase-3 protein expression.
作者信息
Borghetti Gina, Yamaguchi Adriana Aya, Aikawa Julia, Yamazaki Ricardo Key, de Brito Gleisson Alisson Pereira, Fernandes Luiz Claudio
机构信息
Centro de Estudos da Biodiversidade, Universidade Federal de Roraima, Campus Paricarana Avenida Capitão Ene Garcez, 2413, Bairro Aeroporto Cep: 69310-000, Boa Vista, Roraima, Brazil.
Instituto de Pesquisa Pelé Pequeno Príncipe, Hospital Pequeno Príncipe, Curitiba, Paraná, Brasil.
出版信息
Lipids Health Dis. 2015 Aug 25;14:94. doi: 10.1186/s12944-015-0098-y.
BACKGROUND
Several studies have been shown pro-apoptotic effects of fish oil (FO), rich in n-3 polyunsaturated fatty acids (n-3 PUFA) on cancer cells. Nevertheless, few in vivo experiments have provided data of its ability on apoptosis protein expression in tumor tissue. Thus, in this study we investigate the effect of FO supplementation on apoptosis protein expression in Walker 256 tumor bearing rats. Male Wistar rats were randomly assigned to three groups: fed with regular chow (W); fed regular chow supplemented with FO (WFO) or coconut fat (WCO) (1 g/kg body weight/daily). After thirty days, all animals were inoculated subcutaneously with Walker 256 tumor cells.
FINDINGS
Protein expression was done by western blotting in Walker 256 tumor tissue samples. FO decreased the Bcl-2/Bax ratio (p < 0.05) and increased the p53 (p < 0.05), cleaved caspase-7 (p < 0.05) and cleaved caspase-3 (p < 0.05) in Walker 256 tumor tissue.
CONCLUSIONS
Our data suggest that the pro-apoptotic effect of FO in Walker 256 tumor is related with specifics cleaved caspases.
背景
多项研究表明，富含n-3多不饱和脂肪酸（n-3 PUFA）的鱼油（FO）对癌细胞具有促凋亡作用。然而，很少有体内实验提供其对肿瘤组织中凋亡蛋白表达影响的数据。因此，在本研究中，我们调查了补充鱼油对荷Walker 256肿瘤大鼠凋亡蛋白表达的影响。雄性Wistar大鼠被随机分为三组：喂食普通饲料（W）；喂食添加鱼油（WFO）或椰子油（WCO）（1克/千克体重/每日）的普通饲料。三十天后，所有动物均皮下接种Walker 256肿瘤细胞。
研究结果
通过蛋白质印迹法检测Walker 256肿瘤组织样本中的蛋白表达。鱼油降低了Walker 256肿瘤组织中的Bcl-2/Bax比值（p < 0.05），并增加了p53（p < 0.05）、裂解的半胱天冬酶-7（p < 0.05）和裂解的半胱天冬酶-3（p < 0.05）的表达。
结论
我们的数据表明，鱼油对Walker 256肿瘤的促凋亡作用与特定的裂解半胱天冬酶有关。
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