miR-382 通过调控 EMT 抑制卵巢癌细胞迁移和侵袭并靶向 ROR1。

miR-382 inhibits migration and invasion by targeting ROR1 through regulating EMT in ovarian cancer.

机构信息

Department of Pathology, School of Basic Medical Science, Central South University, Changsha, Hunan 410013, P.R. China.

Children's Medical Center, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China.

出版信息

Int J Oncol. 2016 Jan;48(1):181-90. doi: 10.3892/ijo.2015.3241. Epub 2015 Nov 9.

DOI:10.3892/ijo.2015.3241

PMID:26575700

Abstract

Increasing evidence suggests that microRNAs (miRNAs) play a critical role in tumorigenesis. Decreased expression of miR‑382 has been observed in various types of cancers. However, the biological function of miRNA-382 in ovarian cancer is still largely unknown. Here, we found miR‑382 was downregulated in human ovarian cancer tissues and cell lines. miR‑382 inhibited ovarian cancer cell proliferation, migration, invasion and the epithelial-mesenchymal transition (EMT). Furthermore, we identified receptor tyrosine kinase orphan receptor 1 (ROR1) as a target of miR‑382, and miR‑382 rescued the promotion effect of ROR1 on migration, invasion and EMT process in SKOV3 and COV434 cells. Collectively, these findings revealed that miR‑382 inhibits migration and invision by targeting ROR1 through regulating EMT in ovarian cancer, and might serve as a tumor suppressor in ovarian cancer.

摘要

越来越多的证据表明 microRNAs（miRNAs）在肿瘤发生中发挥着关键作用。已经观察到 miR-382 在各种类型的癌症中表达降低。然而，miRNA-382 在卵巢癌中的生物学功能在很大程度上仍然未知。在这里，我们发现 miR-382 在人卵巢癌组织和细胞系中下调。miR-382 抑制卵巢癌细胞增殖、迁移、侵袭和上皮-间充质转化（EMT）。此外，我们确定了受体酪氨酸激酶孤儿受体 1（ROR1）是 miR-382 的靶标，并且 miR-382 挽救了 ROR1 对 SKOV3 和 COV434 细胞迁移、侵袭和 EMT 过程的促进作用。总之，这些发现表明 miR-382 通过调节 EMT 抑制 ROR1 抑制卵巢癌的迁移和侵袭，并且可能作为卵巢癌的肿瘤抑制因子。