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白首乌(萝藦科)的完整叶绿体基因组序列

The complete chloroplast genome sequence of Cynanchum auriculatum Royle ex Wight (Apocynaceae).

作者信息

Jang Woojong, Kim Kyu-Yeob, Kim Kyunghee, Lee Sang-Choon, Park Hyun-Seung, Lee Junki, Seong Rack Seon, Shim Young Hun, Sung Sang Hyun, Yang Tae-Jin

机构信息

a Department of Plant Science , Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University , Seoul , Republic of Korea.

b Herbal Medicine Research Division , National Institute of Food and Drug Safety Evaluation , Cheongwon-Gun, Chungbuk , Republic of Korea.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4549-4550. doi: 10.3109/19401736.2015.1101557. Epub 2015 Dec 7.

Abstract

Cynanchum auriculatum is a climbing vine belonging to the Apocynaceae family and shows very similar morphology to Cynanchum wilfordii, a medicinal plant. The complete chloroplast genome of C. auriculatum was generated by de novo assembly using the small amount of whole genome sequencing data. The chloroplast genome of C. auriculatum was 160 840 bp in length and consisted of four distinct regions, such as large single copy region (91 973 bp), small single copy region (19 667 bp), and a pair of inverted repeat regions (24 600 bp). The overall GC contents of the chloroplast genome were 37.8%. A total of 114 genes were predicted and included 80 protein-coding genes, 30 tRNA genes, and four rRNA genes. Phylogenetic analysis with the reported chloroplast genomes revealed that C. auriculatum is most closely related to Cynanchum wilfordii, a medicinal plant.

摘要

白首乌是一种属于夹竹桃科的攀缘藤本植物,其形态与药用植物何首乌非常相似。利用少量全基因组测序数据进行从头组装,获得了白首乌的完整叶绿体基因组。白首乌的叶绿体基因组长度为160840bp,由四个不同区域组成,即大单拷贝区域(91973bp)、小单拷贝区域(19667bp)和一对反向重复区域(24600bp)。叶绿体基因组的总体GC含量为37.8%。共预测到114个基因,包括80个蛋白质编码基因、30个tRNA基因和4个rRNA基因。与已报道的叶绿体基因组进行系统发育分析表明,白首乌与药用植物何首乌关系最为密切。

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