Prabhudas Sudheesh K, Raju Balaji, Kannan Thodi Shahid, Parani Madasamy, Natarajan Purushothaman
a Department of Genetic Engineering , SRM University , Kattankulathur , Tamil Nadu , India.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4622-4623. doi: 10.3109/19401736.2015.1101586. Epub 2015 Dec 28.
Brassica juncea L. is the second most important edible oil seed crop of India. In the current study, we report the complete chloroplast genome sequence of Indian mustard, Brassica juncea L. The size of the complete chloroplast genome was found to be 153 483 bp long with an overall GC content of 36.36%. A large single copy (LSC) region of 83 286 bp and a short single copy region (SSC) region of 17 775 bp were separated by a pair of inverted repeat (IR) regions of 26 211 bp. A total of 113 unique genes were annotated, which includes 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. Either one or two introns were present in nine protein-coding genes and six tRNA genes. Phylogenetic analysis with the complete chloroplast genomes of other related species revealed that B. juncea is much closer to Brassica rapa.
芥菜(Brassica juncea L.)是印度第二重要的食用油籽作物。在本研究中,我们报道了印度芥菜(Brassica juncea L.)完整的叶绿体基因组序列。完整叶绿体基因组的大小为153483 bp,总体GC含量为36.36%。一个83286 bp的大单拷贝(LSC)区域和一个17775 bp的短单拷贝区域(SSC)被一对26211 bp的反向重复(IR)区域隔开。总共注释了113个独特基因,其中包括79个蛋白质编码基因、30个tRNA基因和4个rRNA基因。9个蛋白质编码基因和6个tRNA基因中存在一个或两个内含子。与其他相关物种的完整叶绿体基因组进行系统发育分析表明,芥菜与白菜型油菜的亲缘关系更近。