用于检测急性呼吸道感染病原体的基于微珠的悬浮阵列的开发。
Development of a bead-based suspension array for the detection of pathogens in acute respiratory tract infections.
作者信息
Chen Yu-Sheng, Li Hong-Ru, Zhang Wei, Hua Zhi-Dan, Lin Xiao-Hong, Lin Meng-Qing, Huang Wen-Sen, Huang Li-Ping, Yu Xiao-Li, Xu Neng-Luan, Lin Ming, Xie Bao-Song, Shen Xiao-Na, Xie Jian-Feng, Wang Yi, Huang Meng, Wu Yan-An, Hu Xin-Lan
机构信息
Department of Respiratory and Critical Care Medicine, Fujian Provicial Hospital, Fujian Provincial Medical College, Fujian Medical University, Fuzhou 350001, China
Department of Respiratory and Critical Care Medicine, Fujian Provicial Hospital, Fujian Provincial Medical College, Fujian Medical University, Fuzhou 350001, China.
出版信息
Exp Biol Med (Maywood). 2016 Aug;241(14):1551-8. doi: 10.1177/1535370216647128. Epub 2016 May 13.
We developed a high-throughput bead-based suspension array for simultaneous detection of 20 respiratory tract pathogens in clinical specimens. Pathogen-specific genes were amplified and hybridized to probes coupled to carboxyl-encoded microspheres. Fluorescence intensities generated via the binding of phycoerythrin-conjugated streptavidin with biotin-labeled targets were measured by the Luminex 100 bead-based suspension array system. The bead-based suspension array detected bacteria in a significantly higher number of samples compared to the conventional culture. There was no significant difference in the detection rate of atypical pathogensatypical pathogens or viruses between the bead-based suspension array and real-time PCR. This technology can play a significant role in screening patients with pneumonia.
我们开发了一种基于微珠的高通量悬浮阵列，用于同时检测临床标本中的20种呼吸道病原体。病原体特异性基因被扩增并与偶联到羧基编码微球上的探针杂交。通过藻红蛋白偶联的链霉亲和素与生物素标记的靶标的结合产生的荧光强度，由基于Luminex 100微珠的悬浮阵列系统进行测量。与传统培养相比，基于微珠的悬浮阵列在更多样本中检测到了细菌。在基于微珠的悬浮阵列与实时PCR之间，非典型病原体或病毒的检测率没有显著差异。这项技术在肺炎患者的筛查中可发挥重要作用。
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