姜黄素和鞣花酸协同诱导人宫颈癌HeLa细胞产生活性氧、DNA损伤、p53蛋白积累及凋亡。

Curcumin and Ellagic acid synergistically induce ROS generation, DNA damage, p53 accumulation and apoptosis in HeLa cervical carcinoma cells.

作者信息

Kumar Devbrat, Basu Soumya, Parija Lucy, Rout Deeptimayee, Manna Sanjeet, Dandapat Jagneshwar, Debata Priya Ranjan

机构信息

Department of Biotechnology, Utkal University, Bhubaneswar, Odisha, India.

Department of Zoology, North Orissa University, Baripada, Mayurbhanj, Odisha, India.

出版信息

Biomed Pharmacother. 2016 Jul;81:31-37. doi: 10.1016/j.biopha.2016.03.037. Epub 2016 Apr 8.

DOI:10.1016/j.biopha.2016.03.037

PMID:27261574

Abstract

Cervical cancer and precancerous lesions of the cervix continue to be a global health issue, and the medication for the treatment for chronic HPV infection so far has not been effective. Potential anticancer and anti HPV activities of two known phytochemicals, Curcumin and Ellagic acid were evaluated in HeLa cervical cancer cells. Curcumin is a natural compound found in the root of Curcuma longa plant and Ellagic acid a polyphenol found in fruits of strawberries, raspberries and walnuts. The combination of Curcumin and Ellagic acid at various concentrations showed better anticancer properties than either of the drug when used alone as evidenced by MTT assay. Besides this, Curcumin and Ellagic acid also restore p53, induce ROS formation and DNA damage. Mechanistic study further indicated that Curcumin and Ellagic acid show anti-HPV activity as evidenced by decrease in the HPV E6 oncoprotein on HeLa cells.

摘要

宫颈癌及宫颈前病变仍是一个全球性的健康问题，并且目前用于治疗慢性人乳头瘤病毒（HPV）感染的药物尚无疗效。在人乳头瘤病毒（HPV）感染的HeLa宫颈癌细胞中评估了两种已知植物化学物质姜黄素和鞣花酸潜在的抗癌及抗HPV活性。姜黄素是一种存在于姜黄植物根部的天然化合物，鞣花酸是一种存在于草莓、树莓及核桃果实中的多酚。MTT法检测表明，不同浓度的姜黄素与鞣花酸联合使用时，其抗癌特性优于单独使用其中任何一种药物。除此之外，姜黄素和鞣花酸还能恢复p53、诱导活性氧（ROS）生成及DNA损伤。机制研究进一步表明，姜黄素和鞣花酸具有抗HPV活性，这在HeLa细胞中HPV E6癌蛋白的减少中得到证实。