来自五位同胞母亲的24个斑马鱼单个鱼卵的母体RNA转录组数据。
Transcriptome data on maternal RNA of 24 individual zebrafish eggs from five sibling mothers.
作者信息
Pagano Johanna F B, Rauwerda Han, de Leeuw Wim C, Wackers Paul, de Jong Mark, Ensink Wim, Dekker Rob, Nehrdich Ulrike, Spaink Herman P, Jonker Martijs, Breit Timo M
机构信息
RNA Biology & Applied Bioinformatics research group, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Amsterdam, The Netherlands.
Institute of Biology Leiden, Faculty of Science, Leiden University, Leiden, The Netherlands.
出版信息
Data Brief. 2016 Apr 26;8:69-72. doi: 10.1016/j.dib.2016.04.045. eCollection 2016 Sep.
Maternal mRNA that is present in the mature oocyte plays an important role in the proper development of the early embryo. To elucidate the role of the maternal transcriptome we recently reported a microarray study on individual zebrafish eggs from five different clutches from sibling mothers and showed differences in maternal RNA abundance between and within clutches, "Mother-specific signature in the maternal transcriptome composition of mature, unfertilized Eggs" [1]. Here we provide in detail the applied preprocessing method as well as the R-code to identify expressed and non-expressed genes in the associated transcriptome dataset. Additionally, we provide a website that allows a researcher to search for the expression of their gene of interest in this experiment.
成熟卵母细胞中存在的母体mRNA在早期胚胎的正常发育中起着重要作用。为了阐明母体转录组的作用，我们最近报道了一项针对来自同胞母亲的五个不同批次的单个斑马鱼卵的微阵列研究，并显示了不同批次之间以及批次内母体RNA丰度的差异，“成熟未受精卵的母体转录组组成中的母体特异性特征”[1]。在这里，我们详细提供了应用的预处理方法以及用于识别相关转录组数据集中表达和未表达基因的R代码。此外，我们提供了一个网站，允许研究人员在该实验中搜索他们感兴趣基因的表达情况。
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