非小细胞肺癌血清和组织中miR-181b-5p和miR-486-5p的异常表达
Aberrant miR-181b-5p and miR-486-5p expression in serum and tissue of non-small cell lung cancer.
作者信息
Tian Fei, Shen Yanting, Chen Zhenzhu, Li Rui, Lu Jiafeng, Ge Qinyu
机构信息
Research Center for Learning Science, Southeast University, Nanjing 210096, China; State Key Lab of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Research Center for Learning Science, Southeast University, Nanjing 210096, China.
出版信息
Gene. 2016 Oct 15;591(2):338-43. doi: 10.1016/j.gene.2016.06.014. Epub 2016 Jun 9.
BACKGROUND
Lung cancer is the leading cause of cancer deaths in China. Non-small cell lung cancer (NSCLC) is the major type of lung cancer.
OBJECTIVES
The aim of our study was to characterize the expression profiles of miRNAs in serum and tissue of NSCLC at the same time, and to find more accurate relationship of miRNAs between serum and tissue. Furthermore, we intended to find more biomarkers of miRNAs in NSCLC samples.
METHODS
In this study, the miRNAs were sequenced in 18 paired serum and 18 paired tissue samples. The expression levels of miRNAs and targets were quantified by qRT-PCR. The function analysis was performed by using bioinformatics methods.
RESULTS
In these paired samples miR-181b-5p was up-regulated in squamous cell carcinoma (SCC), miR-486-5p was down-regulated in adenocarcinoma (AC), and miR-21-5p was up-regulated in both SCC and AC. However, miR-181b-5p and miR-486-5p were rarely reported in lung cancer related studies. The expression levels of these two miRNAs and their targets, RASSF1 and PIK3R1 were quantified in additional samples by qRT-PCR. The results showed that the targets were negatively regulated by the two miRNAs. In addition, we noted that RASSF1 and PIK3R1 were directly involved in non-small cell lung cancer pathway.
CONCLUSIONS
Our study suggested that miR-181b-5p and miR-486-5p could be new potential biomarkers for early diagnosis of NSCLC.
背景
肺癌是中国癌症死亡的主要原因。非小细胞肺癌（NSCLC）是肺癌的主要类型。
目的
本研究旨在同时表征NSCLC患者血清和组织中miRNA的表达谱，并找到血清和组织中miRNA更准确的关系。此外，我们旨在在NSCLC样本中找到更多miRNA生物标志物。
方法
在本研究中，对18对配对的血清和18对配对的组织样本进行miRNA测序。通过qRT-PCR对miRNA及其靶标的表达水平进行定量。使用生物信息学方法进行功能分析。
结果
在这些配对样本中，miR-181b-5p在鳞状细胞癌（SCC）中上调，miR-486-5p在腺癌（AC）中下调，miR-21-5p在SCC和AC中均上调。然而，在肺癌相关研究中很少报道miR-181b-5p和miR-486-5p。通过qRT-PCR在另外的样本中对这两种miRNA及其靶标RASSF1和PIK3R1的表达水平进行定量。结果表明，这两种miRNA对靶标具有负调控作用。此外，我们注意到RASSF1和PIK3R1直接参与非小细胞肺癌通路。
结论
我们的研究表明，miR-181b-5p和miR-486-5p可能是NSCLC早期诊断的新的潜在生物标志物。
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