刺糙叶树（Nees & Mart）Schauer叶片精油对某些抗菌药物的抗菌活性及调节作用
Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs.
作者信息
Duarte Antonia Eliene, de Menezes Irwin Rose Alencar, Bezerra Morais Braga Maria Flaviana, Leite Nadghia Figueiredo, Barros Luiz Marivando, Waczuk Emily Pansera, Pessoa da Silva Maria Arlene, Boligon Aline, Teixeira Rocha João Batista, Souza Diogo Onofre, Kamdem Jean Paul, Melo Coutinho Henrique Douglas, Escobar Burger Marilise
机构信息
Centro de Ciências Biológicas e da Saúde (CC BS), Departamento de Ciências Biológicas, Universidade Regional do Cariri (URCA), Crato 63105-000, CE, Brazil.
Programa de Pós-Graduação em Bioquímica Toxicológica, Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Santa Maria, Santa Maria 97105-900, RS, Brazil.
出版信息
Molecules. 2016 Jun 8;21(6):743. doi: 10.3390/molecules21060743.
BACKGROUND
Rhaphiodon echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. echinus leaf was investigated for its antimicrobial properties.
METHODS
The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined.
RESULTS
The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity.
CONCLUSIONS
The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs.
背景
多刺糙齿草是一种杂草植物，在巴西民间医学中用于治疗传染病。在本研究中，对多刺糙齿草叶的精油进行了抗菌特性研究。
方法
通过气相色谱-质谱联用仪（GC-MS）对精油的化学成分进行表征。通过微量稀释法研究精油单独以及与抗真菌或抗生素药物联合使用时对白色念珠菌、克柔念珠菌和热带念珠菌等真菌以及大肠杆菌、金黄色葡萄球菌和假单胞菌等微生物的作用，从而确定其抗菌特性。此外，还测定了该精油的铁（II）螯合潜力。
结果
结果表明，主要化合物中存在β-石竹烯和双环大根香叶烯，精油显示出较低的抗真菌和抗菌活性，但与精油联合使用时对抗菌药物有很强的调节作用。该精油显示出铁（II）螯合活性。
结论
气相色谱-质谱联用仪（GC-MS）表征显示精油中存在单萜和倍半萜以及金属螯合潜力，这可能部分解释了该精油的调节作用。这些发现表明，多刺糙齿草精油是一种能够增强抗菌药物抗菌和抗真菌活性的天然产物。
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