通过免疫荧光染色和显微镜成像检测石蜡包埋组织切片中的细胞增殖标志物
Detection of Cell Proliferation Markers by Immunofluorescence Staining and Microscopy Imaging in Paraffin-Embedded Tissue Sections.
作者信息
Eminaga Seda, Teekakirikul Polakit, Seidman Christine E, Seidman Jonathan G
机构信息
Cardiovascular Division, King's College London, London, United Kingdom.
Heart and Vascular Institute, UPMC Presbyterian, Pittsburgh, Pennsylvania.
出版信息
Curr Protoc Mol Biol. 2016 Jul 1;115:14.25.1-14.25.14. doi: 10.1002/cpmb.13.
Abstract
This unit describes a step-by-step protocol to detect and quantify proliferating cells in paraffin-embedded tissue sections. Two well-established markers of proliferation (incorporation of BrdU into newly synthesized DNA and expression of the nuclear protein Ki67) are detected after antigen-retrieval and subsequent immunofluorescence staining and confocal microscopy. © 2016 by John Wiley & Sons, Inc.
摘要
本单元描述了一种用于检测和定量石蜡包埋组织切片中增殖细胞的逐步方案。在抗原修复、随后的免疫荧光染色和共聚焦显微镜检查后，检测两种成熟的增殖标志物（将溴脱氧尿苷掺入新合成的DNA以及核蛋白Ki67的表达）。© 2016约翰威立国际出版公司。
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