小鼠大脑中的遗传密码扩展。
Genetic code expansion in the mouse brain.
作者信息
Ernst Russell J, Krogager Toke P, Maywood Elizabeth S, Zanchi Roberto, Beránek Václav, Elliott Thomas S, Barry Nicholas P, Hastings Michael H, Chin Jason W
机构信息
Medical Research Council Laboratory of Molecular Biology, Cambridge, England, UK.
出版信息
Nat Chem Biol. 2016 Oct;12(10):776-778. doi: 10.1038/nchembio.2160. Epub 2016 Aug 29.
Abstract
Site-specific incorporation of non-natural amino acids into proteins, via genetic code expansion with pyrrolysyl tRNA synthetase (PylRS) and tRNA(Pyl)CUA pairs (and their evolved derivatives) from Methanosarcina sp., forms the basis of powerful approaches to probe and control protein function in cells and invertebrate organisms. Here we demonstrate that adeno-associated viral delivery of these pairs enables efficient genetic code expansion in primary neuronal culture, organotypic brain slices and the brains of live mice.
摘要
通过利用来自甲烷八叠球菌属的吡咯赖氨酸tRNA合成酶（PylRS）和tRNA(Pyl)CUA对（及其进化衍生物）进行遗传密码扩展，将非天然氨基酸位点特异性地掺入蛋白质中，这构成了在细胞和无脊椎动物中探测和控制蛋白质功能的强大方法的基础。在这里，我们证明这些对的腺相关病毒递送能够在原代神经元培养物、脑片和活体小鼠大脑中实现有效的遗传密码扩展。
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