Q 系统：一种用于果蝇的通用表达系统。
The Q-System: A Versatile Expression System for Drosophila.
作者信息
Riabinina Olena, Potter Christopher J
机构信息
The Solomon H. Snyder Department of Neuroscience, The Center for Sensory Biology, Johns Hopkins University School of Medicine, 855N. Wolfe St., Baltimore, MD, 21205, USA.
出版信息
Methods Mol Biol. 2016;1478:53-78. doi: 10.1007/978-1-4939-6371-3_3.
Abstract
Binary expression systems are flexible and versatile genetic tools in Drosophila. The Q-system is a recently developed repressible binary expression system that offers new possibilities for transgene expression and genetic manipulations. In this review chapter, we focus on current state-of-the-art Q-system tools and reagents. We also discuss in vivo applications of the Q-system, together with GAL4/UAS and LexA/LexAop systems, for simultaneous expression of multiple effectors, intersectional labeling, and clonal analysis.
摘要
二元表达系统是果蝇中灵活且通用的遗传工具。Q系统是最近开发的一种可抑制的二元表达系统，为转基因表达和基因操作提供了新的可能性。在这篇综述章节中，我们重点关注当前最先进的Q系统工具和试剂。我们还讨论了Q系统与GAL4/UAS和LexA/LexAop系统在体内的应用，用于同时表达多种效应物、交叉标记和克隆分析。
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