表皮生长因子受体-丝裂原活化蛋白激酶（EGFR-MAPK）信号通路在广藿香醇诱导A549细胞凋亡及细胞周期阻滞中的分子作用
Molecular Role of EGFR-MAPK Pathway in Patchouli Alcohol-Induced Apoptosis and Cell Cycle Arrest on A549 Cells and .
作者信息
Lu XinGang, Yang Liu, Lu ChengHua, Xu ZhenYu, Qiu HongFu, Wu JiaJia, Wang JingWen, Tong JiaFeng, Zhu Yin, Shen Jie
机构信息
Department of Traditional Chinese Medicine, Huadong Hospital, Fudan University, Shanghai 200040, China.
Department of Oncology, Baoshan Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201999, China.
出版信息
Biomed Res Int. 2016;2016:4567580. doi: 10.1155/2016/4567580. Epub 2016 Oct 17.
Nowadays, chemotherapy is still the main effective treatment for cancer. Herb prescriptions containing (also known as "Guang-Huo-Xiang") have been widely used in Chinese medicine today. In our research, we found that patchouli alcohol, a compound isolated from the oil of , exerted antitumor ability against human lung cancer A549 cells ability both and . MTT assay was used to assess cell viability. Hoechst 33342 staining and TUNEL cover glass staining provided the visual evidence of apoptosis. Caspase activity measurement showed that patchouli alcohol activated caspase 9 and caspase 3 of mitochondria-mediated apoptosis. Consistently, patchouli alcohol inhibited the xenograft tumor . Further investigation of the underlying molecular mechanism showed that MAPK and EGFR pathway might contribute to the antitumor effect of patchouli alcohol. Our study proved that patchouli alcohol might be able to serve as a novel antitumor compound in the clinical treatment of lung cancer.
如今，化疗仍是癌症的主要有效治疗方法。含有广藿香（也称为“Guang-Huo-Xiang”）的草药方剂如今已在中国医学中广泛使用。在我们的研究中，我们发现从广藿香油中分离出的化合物广藿香醇对人肺癌A549细胞具有抗肿瘤能力，无论是在体外还是体内。MTT法用于评估细胞活力。Hoechst 33342染色和TUNEL盖玻片染色提供了细胞凋亡的可视化证据。半胱天冬酶活性测定表明，广藿香醇激活了线粒体介导的凋亡中的半胱天冬酶9和半胱天冬酶3。同样，广藿香醇抑制了异种移植肿瘤。对潜在分子机制的进一步研究表明，MAPK和EGFR通路可能有助于广藿香醇的抗肿瘤作用。我们的研究证明，广藿香醇可能能够作为一种新型抗肿瘤化合物用于肺癌的临床治疗。
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