一种用于检测和鉴定棒状杆菌中O-分枝菌酰化蛋白的化学报告策略。
A chemical reporter strategy for detecting and identifying O-mycoloylated proteins in Corynebacterium.
作者信息
Kavunja Herbert W, Piligian Brent F, Fiolek Taylor J, Foley Hannah N, Nathan Temitope O, Swarts Benjamin M
机构信息
Department of Chemistry and Biochemistry, Central Michigan University, Mount Pleasant, MI 48859, USA.
出版信息
Chem Commun (Camb). 2016 Nov 22;52(95):13795-13798. doi: 10.1039/c6cc07143k.
PMID:27831572
Abstract
Protein O-mycoloylation is a unique post-translational lipidation that was recently discovered in Corynebacterium. We describe an alkyne-modified trehalose monomycolate chemical reporter that can metabolically tag O-mycoloylated proteins in C. glutamicum, enabling their detection and identification through click chemistry.
摘要
蛋白质O-分枝菌酰化是一种最近在棒状杆菌中发现的独特的翻译后脂化修饰。我们描述了一种炔烃修饰的海藻糖单分枝菌酸化学报告分子，它可以在谷氨酸棒杆菌中对O-分枝菌酰化的蛋白质进行代谢标记，从而通过点击化学实现对它们的检测和鉴定。
Zotero 插件