雷尼霉素M减轻H460肺癌细胞中癌症干细胞样表型。
Renieramycin M Attenuates Cancer Stem Cell-like Phenotypes in H460 Lung Cancer Cells.
作者信息
Sirimangkalakitti Natchanun, Chamni Supakarn, Suwanborirux Khanit, Chanvorachote Pithi
机构信息
Center for Bioactive Natural Products from Marine Organisms and Endophytic Fungi, Department of Pharmacognosy and Pharmaceutical Botany, Chulalongkorn University, Pathumwan, Bangkok, Thailand.
Cell-Based Drug and Health Product Development Research Unit and Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Pathumwan, Bangkok, Thailand
出版信息
Anticancer Res. 2017 Feb;37(2):615-621. doi: 10.21873/anticanres.11355.
BACKGROUND/AIM: Cancer stem cells (CSCs) are a subpopulation of cancer cells that possess self-renewal and differentiation capacities. CSCs contribute to drug-resistance, cancer recurrence and metastasis, thus development of CSC-targeted therapeutic strategies has recently received significant attention in cancer research. In this study, the potential efficacy of renieramycin M (RM) isolated from the sponge Xestospongia species, was examined against lung CSCs.
MATERIALS AND METHODS
Colony and spheroid formation assays, as well as western blotting analysis of lung CSC protein markers were employed to determine the CSC-like phenotypes of H460 lung cancer cells after treatment with RM at non-toxic concentrations.
RESULTS
RM treatment reduced significantly colony and spheroid formation of H460 cells. Moreover, the CSC markers CD133, CD44 and ALDH1A1 of CSC-enriched H460 cells were reduced significantly following RM treatment.
CONCLUSION
RM could be a potent anti-metastatic agent by suppressing lung CSC-like phenotypes in H460 cells.
背景/目的：癌症干细胞（CSCs）是癌细胞的一个亚群，具有自我更新和分化能力。癌症干细胞导致耐药性、癌症复发和转移，因此，针对癌症干细胞的治疗策略的开发最近在癌症研究中受到了广泛关注。在本研究中，检测了从海绵Xestospongia物种中分离出的雷尼霉素M（RM）对肺癌症干细胞的潜在疗效。
材料与方法
采用集落和球体形成试验以及对肺癌症干细胞蛋白标志物进行蛋白质印迹分析，以确定用无毒浓度的RM处理后H460肺癌细胞的癌症干细胞样表型。
结果
RM处理显著减少了H460细胞的集落和球体形成。此外，经RM处理后，富含癌症干细胞的H460细胞的癌症干细胞标志物CD133、CD44和ALDH1A1显著减少。
结论
RM可能是一种有效的抗转移剂，通过抑制H460细胞中的肺癌症干细胞样表型发挥作用。
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