一项新的网格细胞到放置细胞转变机制揭示于内侧嗅皮层Ⅱ层的转基因去极化。
A Novel Mechanism for the Grid-to-Place Cell Transformation Revealed by Transgenic Depolarization of Medial Entorhinal Cortex Layer II.
机构信息
Kavli Institute for Systems Neuroscience and Centre for Neural Computation, Norwegian University of Science and Technology, Olav Kyrres gate 9, 7030 Trondheim, Norway; Institute of Neuroscience, University of Oregon, 1254 University of Oregon, Eugene, OR 97403, USA.
Institute of Neuroscience, University of Oregon, 1254 University of Oregon, Eugene, OR 97403, USA.
出版信息
Neuron. 2017 Mar 22;93(6):1480-1492.e6. doi: 10.1016/j.neuron.2017.03.001.
The spatial receptive fields of neurons in medial entorhinal cortex layer II (MECII) and in the hippocampus suggest general and environment-specific maps of space, respectively. However, the relationship between these receptive fields remains unclear. We reversibly manipulated the activity of MECII neurons via chemogenetic receptors and compared the changes in downstream hippocampal place cells to those of neurons in MEC. Depolarization of MECII impaired spatial memory and elicited drastic changes in CA1 place cells in a familiar environment, similar to those seen during remapping between distinct environments, while hyperpolarization did not. In contrast, both manipulations altered the firing rate of MEC neurons without changing their firing locations. Interestingly, only depolarization caused significant changes in the relative firing rates of individual grid fields, reconfiguring the spatial input from MEC. This suggests a novel mechanism of hippocampal remapping whereby rate changes in MEC neurons lead to locational changes of hippocampal place fields.
内侧隔核皮层 II 层（MECII）和海马体中的神经元的空间感受野分别提示了一般性的和特定于环境的空间图谱。然而，这些感受野之间的关系尚不清楚。我们通过化学遗传受体可逆地操纵 MECII 神经元的活动，并将下游海马体位置细胞的变化与 MEC 神经元的变化进行比较。MECII 的去极化会损害空间记忆，并在熟悉的环境中引起 CA1 位置细胞的剧烈变化，类似于在不同环境之间重新映射时观察到的变化，而超极化则不会。相比之下，这两种操作都改变了 MEC 神经元的放电率，而没有改变它们的放电位置。有趣的是，只有去极化会导致个别栅格场的相对放电率发生显著变化，从而重新配置来自 MEC 的空间输入。这表明了海马体重新映射的一种新机制，即 MEC 神经元的率变化导致海马体位置场的位置变化。
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