Transcriptome-Wide Mapping of N -Methyladenosine Methylome.

N -Methyladenosine (mA) is a prevalent posttranscriptional RNA modification and commonly found in tRNA and rRNA. Very recent works have also demonstrated the prevalence of mA in mammalian mRNA. Hence, high-throughput methods that allow transcriptome-wide mapping of mA will be important for further functional investigations. Here, we describe a technique called "mA-ID-Seq", which is based on mA immunoprecipitation and the inherent ability of mA to stall reverse transcription, to map mA in the transcriptome. Utilizing this technique, highly confident mA peaks can be obtained on a transcriptome-wide level.