用于mRNA表观转录组分析的基于反应性的先进测序方法。

Advanced reactivity-based sequencing methods for mRNA epitranscriptome profiling.

作者信息

Cai Zhihe, Song Peizhe, Yu Kemiao, Jia Guifang

机构信息

Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University Beijing 100871 China

Peking-Tsinghua Center for Life Sciences, Peking University Beijing 100871 China.

出版信息

RSC Chem Biol. 2024 Dec 10;6(2):150-169. doi: 10.1039/d4cb00215f. eCollection 2025 Feb 5.

DOI:10.1039/d4cb00215f

PMID:39759443

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11694185/

Abstract

Currently, over 170 chemical modifications identified in RNA introduce an additional regulatory attribute to gene expression, known as the epitranscriptome. The development of detection methods to pinpoint the location and quantify these dynamic and reversible modifications has significantly expanded our understanding of their roles. This review goes deep into the latest progress in enzyme- and chemical-assisted sequencing methods, highlighting the opportunities presented by these reactivity-based techniques for detailed characterization of RNA modifications. Our survey provides a deeper understanding of the function and biological roles of RNA modification.

摘要

目前，在RNA中发现的170多种化学修饰为基因表达引入了一种额外的调控属性，即“表观转录组”。用于精确确定这些动态可逆修饰的位置并进行定量的检测方法的发展，极大地扩展了我们对其作用的理解。本综述深入探讨了酶促测序法和化学辅助测序法的最新进展，强调了这些基于反应性的技术在详细表征RNA修饰方面所带来的机遇。我们的综述使人们对RNA修饰的功能和生物学作用有了更深入的理解。