Scipion网络工具：通过网络轻松进行冷冻电镜图像处理。
Scipion web tools: Easy to use cryo-EM image processing over the web.
作者信息
Conesa Mingo Pablo, Gutierrez José, Quintana Adrián, de la Rosa Trevín José Miguel, Zaldívar-Peraza Airén, Cuenca Alba Jesús, Kazemi Mohsen, Vargas Javier, Del Cano Laura, Segura Joan, Sorzano Carlos Oscar S, Carazo Jose María
机构信息
Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, Madrid, 28049, Spain.
出版信息
Protein Sci. 2018 Jan;27(1):269-275. doi: 10.1002/pro.3315. Epub 2017 Nov 6.
Abstract
Macromolecular structural determination by Electron Microscopy under cryogenic conditions is revolutionizing the field of structural biology, interesting a large community of potential users. Still, the path from raw images to density maps is complex, and sophisticated image processing suites are required in this process, often demanding the installation and understanding of different software packages. Here, we present Scipion Web Tools, a web-based set of tools/workflows derived from the Scipion image processing framework, specially tailored to nonexpert users in need of very precise answers at several key stages of the structural elucidation process.
摘要
低温条件下通过电子显微镜进行大分子结构测定正在彻底改变结构生物学领域，吸引了大量潜在用户群体。然而，从原始图像到密度图的过程很复杂，在此过程中需要复杂的图像处理套件，通常需要安装和了解不同的软件包。在这里，我们展示了Scipion网络工具，这是一组基于网络的工具/工作流程，源自Scipion图像处理框架，特别为在结构解析过程的几个关键阶段需要非常精确答案的非专业用户量身定制。
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