二氧化硅微米和纳米颗粒对基础和特殊单核细胞功能的尺寸依赖性效应的证据。
Evidence of size-dependent effect of silica micro- and nano-particles on basal and specialized monocyte functions.
作者信息
De Marzi Mauricio C, Saraceno Martín, Mitarotonda Romina, Todone Marcos, Fernandez Marisa, Malchiodi Emilio L, Desimone Martín F
机构信息
Laboratorio de Inmunología, Instituto de Ecología y Desarrollo Sustentable (INEDES) CONICET-UNLu, Departamento de Ciencias Básicas, Universidad Nacional de Luján, Ruta 5 y Avenida Constitución (6700) Luján, Buenos Aires, Argentina.
Universidad de Buenos Aires, IDEHU-CONICET, Facultad de Farmacia y Bioquímica, Cátedra de Inmunología, Junín 956, Piso 4° (1113), Buenos Aires, Argentina.
出版信息
Ther Deliv. 2017 Dec;8(12):1035-1049. doi: 10.4155/tde-2017-0053.
AIM
To analyze the effect of silica particles on monocyte/macrophage functions.
MATERIALS & METHODS: Silica micro- and nanoparticles were obtained by the Stöber method. Their effect on monocyte/macrophage proliferation, activation, membrane integrity and metabolic activity were determined.
RESULTS
Silica particles inhibit cell proliferation while 10 nm nanoparticles (NPs) did not affect it. Similarly, silica particles induced strong cell activation. However, 10 nm NPs do not alter IL-12 or nitrite levels. Furthermore, bigger NPs and microparticles increase cell membrane damage and reduce the number of living cells but smallest NPs (10 and 240 nm) did not.
CONCLUSION
Cell activation properties of silica particles could be useful tools for immune stimulation therapy, while 10 nm NPs would be suitable for molecule transportation.
目的
分析二氧化硅颗粒对单核细胞/巨噬细胞功能的影响。
材料与方法
通过施托伯法获得二氧化硅微米颗粒和纳米颗粒。测定它们对单核细胞/巨噬细胞增殖、活化、膜完整性和代谢活性的影响。
结果
二氧化硅颗粒抑制细胞增殖，而10纳米纳米颗粒对其无影响。同样，二氧化硅颗粒可诱导强烈的细胞活化。然而，10纳米纳米颗粒不会改变白细胞介素-12或亚硝酸盐水平。此外，较大的纳米颗粒和微米颗粒会增加细胞膜损伤并减少活细胞数量，但最小的纳米颗粒（10和240纳米）则不会。
结论
二氧化硅颗粒的细胞活化特性可能是免疫刺激疗法的有用工具，而10纳米纳米颗粒适合用于分子运输。
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