基于芘-吡哆醛级联的三一体式荧光传感器用于选择性检测 Zn(ii)、磷酸氢根和半胱氨酸。

Three-in-one type fluorescent sensor based on a pyrene pyridoxal cascade for the selective detection of Zn(ii), hydrogen phosphate and cysteine.

机构信息

Department of Applied Chemistry, SV National Institute of Technology (SVNIT), Surat-395007, India.

出版信息

Dalton Trans. 2018 Jan 15;47(3):742-749. doi: 10.1039/c7dt04234e.

Abstract

A novel fluorescent receptor L was synthesized by Schiff base condensation of 1-pyrenemethylamine with the vitamin B cofactor pyridoxal. The receptor L is highly selective and sensitive towards Zn ions among other tested metal ions. Upon interaction with Zn, the receptor L showed a distinct fluorescence enhancement at 485 nm due to the excimer formation leading to the fluorescent colour change from blue to bluish-green. Subsequently, when the in situ generated ZnL complex interacted with various anions and amino acids, the addition of HPO and cysteine reinstated the fluorescence of the receptor L due to the demetalation of Zn from the ZnL complex. Accordingly, the receptor L was developed for the highly selective, specific and sensitive detection of three important bioactive analytes, i.e., Zn, HPO and cysteine with a detection limit down to 2.3 × 10 M, 2.18 × 10 M and 1.59 × 10 M, respectively. Additionally, the receptor L was applied to the detection of intracellular Zn ions in live HeLa cells.

摘要

一种新型荧光受体 L 通过 1-芘甲胺与维生素 B 辅酶吡哆醛的席夫碱缩合反应合成。该受体 L 对 Zn 离子具有高度选择性和敏感性，而对其他测试的金属离子则没有。与 Zn 相互作用后，由于形成激基复合物，受体 L 在 485nm 处表现出明显的荧光增强，导致荧光颜色从蓝色变为蓝绿色。随后，当原位生成的 ZnL 配合物与各种阴离子和氨基酸相互作用时，添加 HPO 和半胱氨酸会由于 Zn 从 ZnL 配合物中脱金属而恢复受体 L 的荧光。因此，该受体 L 被开发用于高度选择性、特异性和灵敏地检测三种重要的生物活性分析物，即 Zn、HPO 和半胱氨酸，检测限分别低至 2.3×10-7 M、2.18×10-7 M 和 1.59×10-7 M。此外，该受体 L 还被应用于活 HeLa 细胞内 Zn 离子的检测。