新型玻璃纤维增强复合植入物的生物相容性和骨整合评估：体外和体内研究。

An evaluation of the biocompatibility and osseointegration of novel glass fiber reinforced composite implants: In vitro and in vivo studies.

作者信息

Chan Ya-Hui, Lew Wei-Zhen, Lu Emily, Loretz Thomas, Lu Luke, Lin Che-Tong, Feng Sheng-Wei

机构信息

School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.

School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Division of Prosthodontics, Department of Dentistry, Taipei Medical University Hospital, Taipei, Taiwan.

出版信息

Dent Mater. 2018 Mar;34(3):470-485. doi: 10.1016/j.dental.2017.12.001. Epub 2017 Dec 26.

DOI:10.1016/j.dental.2017.12.001

PMID:29287979

Abstract

OBJECTIVES

The aim of this study was to evaluate the in vitro biocompatibility and in vivo osseointegration of three novel bioactive glass fiber reinforced composite (GFRC) implants and to compare these with metal (Ti6Al4V) implants.

METHODS

The surfaces of these experimental substrates were characterized by scanning electron microscopy (SEM), a 2D profilometer and by contact angle measurement. In vitro biological performance was assessed using MG-63 human osteoblast-like cell morphology, cell proliferation assays and the alkaline phosphatase (ALP) activity testing. Furthermore, in vivo osseointegration performance was examined by installing samples into rabbit femurs and evaluated the results using micro-CT, histology and histomorphometrical analysis; these assessments were carried out after 1, 2, 4 and 8 weeks of healing.

RESULTS

The results showed that moderate surface roughness, moderate hydrophilic exposure and moderate homogenous exposure of bioactive glass fibers were present for all of the GFRC substrates. Furthermore, MG-63 cells, when cultured on all of the GFRC substrates, grew well and exhibited a more differentiated phenotype than cells grown on titanium alloy (Ti6Al4V) substrate. Histological evaluation revealed more newly-formed bone regeneration within the thread of the GFRC implants during the initial healing period. In addition, the novel GFRC implants with a bioactive Bio-fiber structure and glass particles within the epoxy resin matrix showed better bone volume/tissue volume (BV/TV) values at 4 weeks and this was accompanied by bone-implant contact (BIC) values at 8 weeks comparable to the Ti6Al4V group.

SIGNIFICANCE

These findings demonstrated that novel GFRC implants seem to show improved osteogenesis and osseointegration functionality and have potential as a substitute for Ti6Al4V, or other metal-based materials, when used for clinically dental and orthopedic applications.

摘要

目的

本研究旨在评估三种新型生物活性玻璃纤维增强复合材料（GFRC）植入物的体外生物相容性和体内骨整合情况，并将其与金属（Ti6Al4V）植入物进行比较。

方法

通过扫描电子显微镜（SEM）、二维轮廓仪和接触角测量对这些实验底物的表面进行表征。使用MG-63人成骨样细胞形态、细胞增殖测定和碱性磷酸酶（ALP）活性测试来评估体外生物学性能。此外，通过将样品植入兔股骨来检查体内骨整合性能，并使用微型计算机断层扫描（micro-CT）、组织学和组织形态计量分析来评估结果；这些评估在愈合1、2、4和8周后进行。

结果

结果表明，所有GFRC底物均具有适度的表面粗糙度、适度的亲水性暴露和适度的生物活性玻璃纤维均匀暴露。此外，当在所有GFRC底物上培养时，MG-63细胞生长良好，并且比在钛合金（Ti6Al4V）底物上生长的细胞表现出更分化的表型。组织学评估显示，在初始愈合期，GFRC植入物螺纹内有更多新形成的骨再生。此外，在环氧树脂基质中具有生物活性生物纤维结构和玻璃颗粒的新型GFRC植入物在4周时显示出更好的骨体积/组织体积（BV/TV）值，并且在8周时的骨-植入物接触（BIC）值与Ti6Al4V组相当。