DNA甲基化不参与成年果蝇饮食限制诱导的寿命延长。
DNA methylation is not involved in dietary restriction induced lifespan extension in adult Drosophila.
作者信息
Lian Ting, Gaur Uma, Wu Q I, Tu Jianbo, Sun Boyuan, Yang Deying, Fan Xiaolan, Mao Xueping, Yang Mingyao
机构信息
Institute of Animal Genetics and Breeding,Sichuan Agricultural University,Chengdu,Sichuan, 611130,P. R. China.
出版信息
Genet Res (Camb). 2018 Feb 1;100:e1. doi: 10.1017/S0016672317000064.
Dietary restriction (DR) is widely regarded as a viable intervention to extend lifespan and healthspan in diverse organisms. The precise molecular regulatory mechanisms are largely unknown. Epigenetic modifications are not stable upon DR and also keep changing with age. Here, we employed whole genome bisulfite sequencing to determine the DNA methylation changes upon DR in adult Drosophila. Our results indicate that although a low level of DNA methylation exists in the adult Drosophila genome, there is no significant difference in DNA methylation levels upon DR when compared to unrestricted flies. This suggests that other epigenetic components such as histone modifications might be altered by DR.
饮食限制（DR）被广泛认为是一种可行的干预措施，可延长多种生物体的寿命和健康寿命。其精确的分子调控机制在很大程度上尚不清楚。表观遗传修饰在饮食限制时并不稳定，并且还会随着年龄的增长而不断变化。在这里，我们采用全基因组亚硫酸氢盐测序来确定成年果蝇在饮食限制后的DNA甲基化变化。我们的结果表明，尽管成年果蝇基因组中存在低水平的DNA甲基化，但与未受限制的果蝇相比，饮食限制后的DNA甲基化水平没有显著差异。这表明其他表观遗传成分，如组蛋白修饰，可能会受到饮食限制的影响而发生改变。
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