Molecular identification and antifungal susceptibility profile of species isolated from patients with vulvovaginitis in Tehran, Iran.

BACKGROUND

Rapid and accurate identification and evaluation of antifungal susceptibility pattern of isolates are crucial to determine suitable antifungal drugs for the treatment of patients with vulvovaginitis candidiasis.

MATERIALS AND METHODS

Vaginal samples were collected from 150 women with suspicious vaginal candidiasis, and then cultured on Sabouraoud's Dextrose Agar with chloramphenicol to isolate species. After identification of isolates using polymerase chain reaction-restriction fragment length polymorphism technique, antifungal susceptibility testing of four azolic antifungal drugs was carried out using broth microdilution method according to the CLSI M27-A3.

RESULTS

species were isolated from eighty suspected patients (61.79%). The most common pathogen was (63.75%). Resistance to fluconazole and ketoconazole was observed in 27.5% and 23.75% of isolates, respectively, and only 2% of isolates were resistant to miconazole. Interestingly, resistance to fluconazole in was more than other species.

CONCLUSION

The results indicated that therapy should be selected according to the antifungal susceptibility tests for the prevention of treatment failure and miconazole therapy can be considered as the best therapeutic choice in the management of vulvovaginitis.