The Effect of Bark on Oral Candidiasis Caused by and Isolated from a Pediatric Oral Infection as Comparison to Azole Antifungal.

BACKGROUND

The original diversity of L. (oak) is in eastern America and then distributed to European districts. Oral candidiasis is the most common fungal infection among humans. Azole antifungal drugs can be used to treat Candida infection. and have emerged as the most common pathogenic yeasts in cases of oral candidiasis.

AIM OF THE STUDY

This study aimed to explore the genotype spp. and evaluate the antifungal activity of hot water extract of oak bark against and as an alternative pharmacotherapy compared to azole antifungal agents.

MATERIALS AND METHODS

The sample was isolated from an 8-year-old child with aggressive oral candidiasis and identified by culturing on sabouraud dextrose agar (SDA) CHROMagar. Genotyping of spp. was performed using the internal transcribed spacer (ITS) region. Standard discs of the antifungal's fluconazole, itraconazole, ketoconazole 10 mg/L for each, amphotericin 100, nystatin 50 mg/L, and hot water oak bark extract were administered to and in vitro.

RESULTS

Genotyping of spp. showed that 98% of oral candidiasis cases were which had an 870 bp genotype, while 2% were which had a 550 bp genotype based on ITS barcoding region size. The findings that the oak bark extract had high antifungal activity against showed an inhibition zone diameter of 3.067 mm compared to high resistance to antifungals.

CONCLUSION

Oak extract is considered a successful alternative for the treatment of oral candidiasis infections by antifungals such as azole and nystatin in children.