Analysis of CRISPR-Cas System in and Its Application.

CRISPR-Cas (Clustered regularly interspaced short palindromic repeats-CRISPR associated proteins) loci, which provide a specific immunity against exogenous elements, are hypervariable among distinct prokaryotes. Based on previous researches, this review focuses on concluding systematical genome editing protocols in Firstly, its protocols and optimized conditions in gene editing are introduced. What's more, classification and diversity analyses of CRISPR-Cas benefit the further understanding of evolution relationship among Ability of its foreign segment integration and spacer source analyses also indicate a new direction of phage resistance. Above all, all of these point out its potential to be regarded as another model system other than type II CRISPR-Cas in .