阿拉伯联合酋长国肠杆菌科分离株中[具体内容缺失]的首次报告
First Report of in Enterobacteriaceae Isolates in the United Arab Emirates.
作者信息
Alfaresi Mubarak, Kim Sing Garwin, Senok Abiola
机构信息
College of Medicine, University of Sharjah, Sharjah, UAE.
Department of Microbiology and Immunology, College of Medicine, Alfaisal University, Riyadh, Saudi Arabia.
出版信息
J Pathog. 2018 Jan 18;2018:1304793. doi: 10.1155/2018/1304793. eCollection 2018.
BACKGROUND
The CTX-M family of extended-spectrum beta lactamase (ESBL) enzymes is comprised of over 60 gene variants with the predominance of in many regions. In this report, we present the first description of in the United Arab Emirates.
METHODS
Forty-five non-duplicate ESBL producing isolates identified in a secondary care facility in the United Arab Emirates from June to July 2016 were studied. Gene sequencing was performed and DNA sequences were annotated using the BLAST program to identify the gene subtypes.
RESULTS
The majority of the ESBL positive isolates were (/ = 39/45; 86.6%) followed by ( = 5) and ( = 1). All isolates harboured and genes, 18 had , and 2 were positive. Thirty-seven isolates (82.2%) were positive for . Other genes identified include ( = 2; isolates #1 and 26) and one each for , , and . No was identified. The predominant subtype was ( = 8) followed by one of each of , , and . The subtypes were and .
CONCLUSION
The findings indicate the first description of in a setting where was previously predominant.
背景
超广谱β-内酰胺酶（ESBL）的CTX-M家族由60多种基因变体组成，在许多地区占主导地位。在本报告中，我们首次描述了阿拉伯联合酋长国的CTX-M。
方法
对2016年6月至7月在阿拉伯联合酋长国一家二级医疗机构中鉴定出的45株非重复产ESBL菌株进行了研究。进行了基因测序，并使用BLAST程序对DNA序列进行注释以鉴定基因亚型。
结果
大多数ESBL阳性菌株为CTX-M-15（39/45；86.6%），其次是CTX-M-14（5株）和CTX-M-27（1株）。所有菌株都携带blaTEM和blaSHV基因，18株有blaCTX-M，2株blaOXA-1阳性。37株菌株（82.2%）blaCTX-M-15阳性。鉴定出的其他blaCTX-M基因包括CTX-M-2（2株；菌株#1和26）以及各1株的CTX-M-9、CTX-M-22和CTX-M-65。未鉴定出blaCTX-M-25。主要的CTX-M亚型是CTX-M-15（8株），其次是CTX-M-14、CTX-M-27和CTX-M-65各1株。blaOXA-1亚型是OXA-1和OXA-10。
结论
这些发现表明在先前以CTX-M-15为主的环境中首次描述了CTX-M-14。
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